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ZIRC
ZFIN ID: ZDB-FIG-171206-57
Liu et al., 2017 - Deficient Cholesterol Esterification in Plasma of apoc2 Knockout Zebrafish and Familial Chylomicronemia Patients. PLoS One   12:e0169939 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Adult

Fig. 3

Expression of lcat, but not cetp, is significantly decreased in apoc2 mutant zebrafish.

In situ hybridization (A) and qPCR (B) results showing lcat and cetp mRNA expression in 5.3 dpf zebrafish embryos. mRNA expression of apoc2, lcat and cetp (C) and apoa1, apoe, apob and mtp (D) in adult zebrafish liver. Results are mean±s.e.m.; numbers of biological replicates are indicated on the graphs; *P<0.05, **P<0.01 and ***P<0.001 (Student’s t-test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
apoa1a AB standard conditions Adult liver RTPCR
apoc2sd38/sd38(AB) standard conditions Adult liver RTPCR
apoba AB standard conditions Adult liver RTPCR
apoc2sd38/sd38(AB) standard conditions Adult liver RTPCR
apoeb AB standard conditions Adult liver RTPCR
apoc2sd38/sd38(AB) standard conditions Adult liver RTPCR
cetp AB standard conditions Day 5 liver ISH
Day 5 whole organism RTPCR
Adult liver RTPCR
apoc2sd38/sd38(AB) standard conditions Day 5 liver ISH
Day 5 whole organism RTPCR
Adult liver RTPCR
lcat AB standard conditions Day 5 liver ISH
Day 5 whole organism RTPCR
Adult liver RTPCR
apoc2sd38/sd38(AB) standard conditions Day 5 liver ISH
Day 5 whole organism RTPCR
Adult liver RTPCR
mttp AB standard conditions Adult liver RTPCR
apoc2sd38/sd38(AB) standard conditions Adult liver RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apoc2sd38/sd38(AB) standard conditions Day 5 liver lcat expression decreased amount, abnormal
apoc2sd38/sd38(AB) standard conditions Day 5 whole organism lcat expression decreased amount, abnormal
apoc2sd38/sd38(AB) standard conditions Adult liver apoa1a expression decreased amount, abnormal
apoc2sd38/sd38(AB) standard conditions Adult liver lcat expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One