FIGURE

Fig. 2

ID

ZDB-FIG-170921-35

Publication

Kara et al., 2017 - miR-27 regulates chondrogenesis by suppressing Focal Adhesion Kinase during pharyngeal arch development

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Fig. 2

Early cranial neural crest (CNC) cell specification and migration are not affected in miR-27 morphants. (A) Lateral view of Tg(sox10(7.2):mRFP)vu234 embryos injected either with MO-ctl or MO27 showing cranial neural crest cell streams populating the pharyngeal arches at 18hpf and 24hpf. Anterior is to the left and posterior is to the right. Neural crest streams are numbered from 1 to 3. The indicated ratio represents the number of embryos with the represented phenotype/total number of observed embryos. (B,C) Expression of the neural crest cell marker, <\sox10\, and CNC marker dlx2a in MO-ctl and MO27 embryos at 16 hpf and 22 hpf detected by whole-mount in situ hybridization. Dorsal view of embryos with anterior side to the top

Expression Data

Genes:	dlx2a mRFP sox10
Fish:	AB AB + MO2-mir27a vu234Tg vu234Tg + MO2-mir27a
Knockdown Reagent:	MO2-mir27a
Anatomical Term:	pharyngeal arch cranial neural crest
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-mir27a vu234Tg + MO2-mir27a
Knockdown Reagent:	MO2-mir27a
Observed In:	pharyngeal arch neural crest cell fate specification pharyngeal arch neural crest cell migration
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 429(1), Kara, N., Wei, C., Commanday, A.C., Patton, J.G., miR-27 regulates chondrogenesis by suppressing Focal Adhesion Kinase during pharyngeal arch development, 321-334, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.