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ZFIN ID: ZDB-PUB-170621-12
miR-27 regulates chondrogenesis by suppressing Focal Adhesion Kinase during pharyngeal arch development
Kara, N., Wei, C., Commanday, A.C., Patton, J.G.
Date: 2017
Source: Developmental Biology   429(1): 321-334 (Journal)
Registered Authors: Patton, James G.
Keywords: chondrogenesis, focal adhesion kinase, miR-27, pharyngeal arches, zebrafish
MeSH Terms:
  • Animal Fins/embryology
  • Animal Fins/metabolism
  • Animals
  • Branchial Region/embryology*
  • Branchial Region/enzymology*
  • Cartilage/pathology
  • Cell Differentiation/genetics
  • Cell Proliferation/genetics
  • Cell Survival/genetics
  • Chondrogenesis/genetics*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Focal Adhesion Protein-Tyrosine Kinases/metabolism*
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Morphogenesis/genetics
  • Neural Crest/cytology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed: 28625871 Full text @ Dev. Biol.
Cranial neural crest cells are a multipotent cell population that generate all the elements of the pharyngeal cartilage with differentiation into chondrocytes tightly regulated by temporal intracellular and extracellular cues. Here, we demonstrate a novel role for miR-27, a highly enriched microRNA in the pharyngeal arches, as a positive regulator of chondrogenesis. Knock down of miR-27 led to nearly complete loss of pharyngeal cartilage by attenuating proliferation and blocking differentiation of pre-chondrogenic cells. Focal adhesion kinase (FAK) is a key regulator in integrin-mediated extracellular matrix (ECM) adhesion and has been proposed to function as a negative regulator of chondrogenesis. We show that FAK is downregulated in the pharyngeal arches during chondrogenesis and is a direct target of miR-27. Suppressing the accumulation of FAK in miR-27 morphants partially rescued the severe pharyngeal cartilage defects observed upon knock down of miR-27. These data support a crucial role for miR-27 in promoting chondrogenic differentiation in the pharyngeal arches through regulation of FAK.