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ZFIN ID: ZDB-FIG-170911-6
Santos-Ledo et al., 2017 - Kinesin-1 promotes chondrocyte maintenance during skeletal morphogenesis. PLoS Genetics   13:e1006918 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Pec-fin to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Pec-fin to Day 5

Fig. 2

Loss of kif5B impairs chondrocyte secretion.

A-C) confocal images of Wt (A), single kif5Baae12/ae12 mutants (B) and kif5Baae12/ae12Kif5Bbae24/ae24 (C) at 60 hpf. Chondrocytes (Sox10:GFP positive cells) secrete proteins positive for peanut agglutinin (PNA, blue) homogeneously. Secretion in single (B) and compound (C) mutants is deficient. Stacking defects are evident in kif5Baae12/ae12Kif5Bbae24/ae24 (C). D-F) Confocal images of Wt (D), single kif5Baae12/ae12 mutants (E) and kif5Baae12/ae12Kif5Bbae24/ae24 (F) at 5 dpf. In Wt (D) Di0C6 (blue) forms variably-sized cellular aggregates. Wheat Germ agglutinin (WGA, green) labels secreted proteins and II-II6B3 marks Collagen II (red) an abundant protein in cartilage. Both are homogeneously secreted in Wt and overlap within ECM (insets in D), although some overlap occurs between Collagen II and Di0C6 (insets in D). In single (E) and compound (F) mutants, Di0C6 forms large patches. Secretion is polarized with little colocalization within ECM (insets in E and F), large patches of Di0C6 colocalize with Collagen II, and cell stacking is perturbed (E, F). Scale bar: 50μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ba2Tg standard conditions Pec-fin mandibular arch skeleton chondrocyte IHC
ba2Tg ; kif5baae12/ae12 standard conditions Pec-fin mandibular arch skeleton chondrocyte IHC
kif5bbae24/ae24 ; kif5baae12/ae12 ; ba2Tg standard conditions Pec-fin mandibular arch skeleton chondrocyte IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC WT standard conditions Day 5 chondrocyte extracellular matrix
IHC kif5baae12/ae12 standard conditions Day 5 chondrocyte extracellular matrix
IHC kif5bbae24/ae24 ; kif5baae12/ae12 standard conditions Day 5 chondrocyte extracellular matrix
Phenotype Details
Fish Conditions Stage Phenotype
ba2Tg ; kif5baae12/ae12 standard conditions Pec-fin chondrocyte secretion decreased occurrence, abnormal
kif5baae12/ae12 standard conditions Day 5 chondrocyte endoplasmic reticulum position, abnormal
kif5baae12/ae12 standard conditions Day 5 chondrocyte extracellular matrix ab1-col2a labeling spatial pattern, abnormal
kif5baae12/ae12 standard conditions Day 5 chondrocyte mitochondrion position, abnormal
kif5baae12/ae12 standard conditions Day 5 chondrocyte secretion decreased occurrence, abnormal
kif5baae12/ae12 standard conditions Day 5 mandibular arch skeleton chondrocyte position, abnormal
kif5bbae24/ae24 ; kif5baae12/ae12 standard conditions Day 5 chondrocyte endoplasmic reticulum position, abnormal
Day 5 chondrocyte extracellular matrix ab1-col2a labeling spatial pattern, abnormal
Day 5 chondrocyte mitochondrion position, abnormal
Day 5 chondrocyte secretion decreased occurrence, abnormal
Day 5 mandibular arch skeleton chondrocyte position, abnormal
kif5bbae24/ae24 ; kif5baae12/ae12 ; ba2Tg standard conditions Pec-fin chondrocyte secretion decreased occurrence, abnormal
Pec-fin mandibular arch skeleton chondrocyte position, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.