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ZFIN ID: ZDB-FIG-170911-8
Santos-Ledo et al., 2017 - Kinesin-1 promotes chondrocyte maintenance during skeletal morphogenesis. PLoS Genetics   13:e1006918 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 4

kif5Blof defects and interactions with Wnt PCP components.

A-F) Semi-thin sections of the trabecular (A, B) and palatoquadrate cartilage (C-F). No differences were detected at 48 hpf (A, B) or 60 hpf (C, D). At 3 dpf, in Wt (E) chondrocytes stack like coins and span the cartilage. However, kif5Blof chondrocytes are circular (F), and close to the cartilage border. Some cells are delineated with dashed outlines and in E-F the cartilage limit is drawn. G-I) Analysis of MTOC orientation using γ-Tubulin. In Wt embryos (G, G´) >80% were anteriorly oriented (I). MTOC position was random in kif5Blof mutants (H, H'). WT: n = 8; 72cells. kif5Blof: n = 5; 63 cells (I). J-K''') Confocal images of single kny (J-J''') or triple mutants (K-K''') at 5 dpf. Secretion is normal in single kny mutants (J'-J''') but stacking is defective (J'''). Stacking and secretion defects in triple mutants (K-K'''). L-M''') Confocal images of single ppt (L-L''') or triple mutants (M-M'''). Secretion is intact in ppt single mutants (L'-L''') but stacking is disrupted (L'''). Membrane (M), secretion (M', M'') and stacking (M''') deficits are evident in triple mutants. ch: ceratohyal; m: Meckel´s; pq: palatoquadrate. Scale bars: A-F: 50μm; G, H: 20μm, J-M''': 50μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ba2Tg standard conditions Protruding-mouth chondrocyte IHC
kif5bbae24/ae24 ; kif5baae12/ae12 ; ba2Tg standard conditions Protruding-mouth chondrocyte IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC gpc4fr6/fr6 standard conditions Day 5 chondrocyte extracellular matrix
IHC kif5baae12/ae12 ; kif5bbae24/ae24 ; gpc4fr6/fr6 standard conditions Day 5 chondrocyte extracellular matrix
IHC kif5bbae24/ae24 ; wnt5bta98/ta98 ; kif5baae12/ae12 standard conditions Day 5 chondrocyte extracellular matrix
IHC wnt5bta98/ta98 standard conditions Day 5 chondrocyte extracellular matrix
Ab1-tubg1 IHC ba2Tg standard conditions Protruding-mouth chondrocyte centrosome
IHC kif5bbae24/ae24 ; kif5baae12/ae12 ; ba2Tg standard conditions Protruding-mouth chondrocyte centrosome
Phenotype Details
Fish Conditions Stage Phenotype
gpc4fr6/fr6 standard conditions Day 5 mandibular arch skeleton chondrocyte morphology, abnormal
gpc4fr6/fr6 standard conditions Day 5 mandibular arch skeleton chondrocyte position, abnormal
kif5baae12/ae12 ; kif5bbae24/ae24 ; gpc4fr6/fr6 standard conditions Day 5 chondrocyte extracellular matrix ab1-col2a labeling spatial pattern, abnormal
Day 5 chondrocyte secretion decreased occurrence, abnormal
Day 5 mandibular arch skeleton decreased size, abnormal
Day 5 mandibular arch skeleton chondrocyte morphology, abnormal
Day 5 mandibular arch skeleton chondrocyte position, abnormal
kif5bbae24/ae24 ; kif5baae12/ae12 standard conditions Protruding-mouth palatoquadrate cartilage chondrocyte circular, abnormal
Protruding-mouth palatoquadrate cartilage chondrocyte position, abnormal
kif5bbae24/ae24 ; kif5baae12/ae12 ; ba2Tg standard conditions Protruding-mouth chondrocyte centrosome polarity, abnormal
Protruding-mouth chondrocyte centrosome position, abnormal
kif5bbae24/ae24 ; wnt5bta98/ta98 ; kif5baae12/ae12 standard conditions Day 5 chondrocyte extracellular matrix ab1-col2a labeling spatial pattern, abnormal
Day 5 chondrocyte secretion decreased occurrence, abnormal
Day 5 mandibular arch skeleton decreased size, abnormal
Day 5 mandibular arch skeleton chondrocyte morphology, abnormal
Day 5 mandibular arch skeleton chondrocyte position, abnormal
wnt5bta98/ta98 standard conditions Day 5 mandibular arch skeleton chondrocyte morphology, abnormal
Day 5 mandibular arch skeleton chondrocyte position, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.