ZFIN ID: ZDB-FIG-170808-19
DeRossi et al., 2017 - MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 3

p53 is downstream of Mpi.

(A) Representative phenotypes of 4 dpf zebrafish larvae following injections of std MO, mpi MO and p53 MO. mpi morphants are characterized by a small head, microphthalmia, pericardial edema, jaw defects and reduced liver size. Examples of normal, mild and severe phenotypes are shown. Co-injection of mpi MO and p53 MO, and injection of mpi MO into p53 MT larvae showed phenotypic rescue of mpi knockdown. (B) The cell death phenotype in mpi morphants, visualized by acridine orange staining, was reversed by co-injection of mpi and p53 morpholino. (C) Quantification of scoring of the phenotypes at 4 dpf, showing that 82% of mpi morphants injected with p53 MO, and 93% of p53 MT injected with mpi MO, were rescued to normal as compared with 22% normal in mpi MO larvae. p-values based on two-tailed Fisher’s exact test. (D) Western blot analysis showing that increase in p53 protein expression in mpi morphants was reduced by co-injection with p53 morpholino. Representative image of four separate clutches. (E) Mpi activity was not affected in either p53 MO co-injected embryos or with mpi MO in the p53 MT. p-Value based on two-tailed paired Student's t-test and Bonferroni correction was applied with alpha = 0.025; N.S. represents p-value>0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tp53 AB control Day 4 whole organism WB
AB + MO1-mpi standard conditions Day 4 whole organism WB
AB + MO1-mpi + MO4-tp53 standard conditions Day 4 whole organism WB
AB + MO4-tp53 standard conditions Day 4 whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-mpi standard conditions Prim-5 apoptotic process increased occurrence, abnormal
Day 4 eye decreased size, abnormal
Day 4 head decreased size, abnormal
Day 4 liver decreased size, abnormal
Day 4 mandibular arch skeleton morphology, abnormal
Day 4 pericardium edematous, abnormal
Day 4 whole organism decreased life span, abnormal
Day 4 whole organism tp53 expression increased amount, abnormal
AB + MO1-mpi + MO4-tp53 standard conditions Prim-5 apoptotic process process quality, ameliorated
Day 4 eye size, ameliorated
Day 4 head size, ameliorated
Day 4 liver size, ameliorated
Day 4 mandibular arch skeleton morphology, ameliorated
Day 4 pericardium edematous, ameliorated
Day 4 whole organism tp53 expression amount, ameliorated
Day 4 whole organism life span, ameliorated
tp53zdf1/zdf1 + MO1-mpi standard conditions Day 4 eye size, ameliorated
Day 4 head size, ameliorated
Day 4 liver size, ameliorated
Day 4 mandibular arch skeleton morphology, ameliorated
Day 4 pericardium edematous, ameliorated
Day 4 whole organism life span, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife