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ZFIN ID: ZDB-FIG-170427-5
Han et al., 2014 - Destabilizing LSD1 by Jade-2 promotes neurogenesis: an antibraking system in neural development. Molecular Cell   55:482-94 Full text @ Mol. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 75%-epiboly

Fig. 6

The Jade-2-LSD1 Pathway Is Implicated in Nervous System Development

(A) The indicated electroporated cortical slices were analyzed by IF microscopy for βIII-tubulin or Nestin. The images of VZs and/or IZs are shown. Scale bar represents 20 μm. The percentage of GFP+/βIII-tubulin+, or GFP+/Nestin+ cells to GFP+ cells was quantified by ImageJ. Slices from two independent experiments were processed for each experimental condition. The results are mean ± SEM of three sections from each experiment. p < 0.05 and ∗∗p < 0.01 (two-tailed unpaired Student’s t test). The images of “shCTR” refer to that in Figure 1E, given that the experiments with shLsd1, shJade-2, or shJade-2+shLsd1 were compared to the same control.

(B) Ten nanograms of each of the indicated morpholinos were injected into one-cell stage embryos before the mRNA level of sox2, sox3, otx2, or hoxb1b was examined at 75% epiboly stage by whole-mount in situ hybridization. Lateral view of dorsal to the right is shown. Scale bar represents 200 μm. The number of observed live embryos (n) was quantified and the ratio of the affected embryos is shown.

See also Figure S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hoxb1b TU control 75%-epiboly neuroectoderm ISH
TU + MO2-jade2 standard conditions 75%-epiboly neuroectoderm ISH
TU + MO2-jade2 + MO5-kdm1a standard conditions 75%-epiboly neuroectoderm ISH
TU + MO5-kdm1a standard conditions 75%-epiboly neuroectoderm ISH
otx2b TU control 75%-epiboly neuroectoderm ISH
TU + MO2-jade2 standard conditions 75%-epiboly neuroectoderm ISH
TU + MO2-jade2 + MO5-kdm1a standard conditions 75%-epiboly neuroectoderm ISH
TU + MO5-kdm1a standard conditions 75%-epiboly neuroectoderm ISH
sox2 TU control 75%-epiboly neuroectoderm ISH
TU + MO2-jade2 standard conditions 75%-epiboly neuroectoderm ISH
TU + MO2-jade2 + MO5-kdm1a standard conditions 75%-epiboly neuroectoderm ISH
TU + MO5-kdm1a standard conditions 75%-epiboly neuroectoderm ISH
sox3 TU control 75%-epiboly neuroectoderm ISH
TU + MO2-jade2 standard conditions 75%-epiboly neuroectoderm ISH
TU + MO2-jade2 + MO5-kdm1a standard conditions 75%-epiboly neuroectoderm ISH
TU + MO5-kdm1a standard conditions 75%-epiboly neuroectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO2-jade2 standard conditions 75%-epiboly neuroectoderm otx2b expression decreased distribution, abnormal
75%-epiboly neuroectoderm hoxb1b expression decreased distribution, abnormal
75%-epiboly neuroectoderm sox3 expression decreased distribution, abnormal
75%-epiboly neuroectoderm sox2 expression decreased distribution, abnormal
TU + MO2-jade2 + MO5-kdm1a standard conditions 75%-epiboly neuroectoderm otx2b expression spatial pattern, ameliorated
75%-epiboly neuroectoderm sox3 expression spatial pattern, ameliorated
75%-epiboly neuroectoderm hoxb1b expression spatial pattern, ameliorated
75%-epiboly neuroectoderm sox2 expression spatial pattern, ameliorated
TU + MO5-kdm1a standard conditions 75%-epiboly neuroectoderm sox2 expression increased distribution, abnormal
75%-epiboly neuroectoderm hoxb1b expression increased distribution, abnormal
75%-epiboly neuroectoderm sox3 expression increased distribution, abnormal
75%-epiboly neuroectoderm otx2b expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol. Cell