ZFIN ID: ZDB-FIG-161221-19
Yu et al., 2016 - Eyes shut homolog is required for maintaining the ciliary pocket and survival of photoreceptors in zebrafish. Biology Open   5(11):1662-1673 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Days 30-44 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 45-89 to Adult

Fig. 6

Rod photoreceptors degenerate in older EYS-deficient zebrafish. (A-F) Retinal sections were stained with Zpr3 and counter stained with DAPI. (A,B) Zpr3 staining of wild-type and EYS-deficient zebrafish at 40 dpf. (C,D) Zpr3 staining of wild-type and EYS-deficient zebrafish at 8 mpf. (E,F) Zpr3 staining of wild-type and EYS-deficient zebrafish at 14 mpf. (G) Quantification of Zpr3 immunofluorescence intensity of wild-type and EYS-deficient zebrafish at 14 mpf. (H,I) Rod quantification in wild-type and EYS-deficient zebrafish at 14 mpf. Rod cells in dorsal and ventral retina near the optic nerve heads were counted on H&E stained sections from sex- and body weight-matched animals. (J,K) GNAT1 western blot and quantification at 14 mpf. Western blot was carried out with GNAT1 and α-tubulin antibodies. Note that no significant differences were observed between wild-type and EYS-deficient zebrafish at 40 dpf and 8 mpf. However, Zpr3 immunoreactivity, rod cell numbers and GNAT1 protein were reduced in EYS-deficient zebrafish at 14 mpf. Scale bar for A-F: 20 µm. Error bars in G,H,K indicate s.e.m.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gnat1 WT control Days 45-89 retina RTPCR
Adult retina RTPCR
eyssny10/sny10 standard conditions Days 45-89 retina RTPCR
Adult retina RTPCR
eyssny14/sny14 standard conditions Days 45-89 retina RTPCR
Adult retina RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-3 IHC WT control Days 30-44 retinal rod cell
IHC Adult retinal rod cell
IHC eyssny10/sny10 standard conditions Days 30-44 retinal rod cell
IHC Adult retinal rod cell
IHC eyssny14/sny14 standard conditions Days 30-44 retina
IHC Days 30-44 retinal rod cell
IHC Adult retina
IHC Adult retinal rod cell
Phenotype Details
Fish Conditions Stage Phenotype
eyssny10/sny10 standard conditions Days 45-89 retinal rod cell normal amount, normal
Adult retina gnat1 expression decreased amount, abnormal
Adult retinal rod cell decreased amount, abnormal
Adult retinal rod cell zpr-3 labeling decreased amount, abnormal
eyssny14/sny14 standard conditions Days 45-89 retinal rod cell normal amount, normal
eyssny14/sny14 standard conditions Adult retina gnat1 expression decreased amount, abnormal
eyssny14/sny14 standard conditions Adult retinal rod cell zpr-3 labeling decreased amount, abnormal
eyssny14/sny14 standard conditions Adult retinal rod cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open