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ZIRC
ZFIN ID: ZDB-FIG-161221-17
Yu et al., 2016 - Eyes shut homolog is required for maintaining the ciliary pocket and survival of photoreceptors in zebrafish. Biology Open   5(11):1662-1673 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Term:
Stage Range: Days 30-44 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 4

EYS-deficient zebrafish exhibited degeneration of cone photoreceptors. Zebrafish retinal sections were immunostained with GNAT2, 1D4, green opsin, blue opsin, and UV opsin antibodies. (A-E′) GNAT2, 1D4, green opsin, blue opsin, and UV opsin staining of 40 dpf wild-type and EYS-deficient zebrafish. There were no apparent differences between wild-type and EYS-deficient zebrafish. (F-J′) GNAT2, 1D4, green opsin, blue opsin, and UV opsin staining of 6 mpf wild-type and EYS-deficient zebrafish. Note GNAT2-, 1D4-, green opsin-, blue opsin-, and UV opsin-positive immunoreactivity was reduced in EYS-deficient zebrafish. (K-O′) GNAT2, 1D4, green opsin, blue opsin, and UV opsin staining of 8 mpf wild-type and EYS-deficient zebrafish. Note that GNAT2-, 1D4-, green opsin-, blue opsin-, and UV opsin-positive immunoreactivity was further reduced in EYS-deficient zebrafish. Scale bar: 20 µm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-opn1mw1 IHC WT control Days 30-44 retina
IHC Adult retina
IHC eyssny10/sny10 standard conditions Days 30-44 retina
IHC Adult retina
IHC eyssny14/sny14 standard conditions Days 30-44 retina
IHC Adult retina
Ab1-opn1sw1 IHC WT control Days 30-44 retina
IHC Adult retina
IHC eyssny10/sny10 standard conditions Days 30-44 retina
IHC Adult retina
IHC eyssny14/sny14 standard conditions Days 30-44 retina
IHC Adult retina
Ab1-opn1sw2 IHC WT control Days 30-44 retina
IHC Adult retina
IHC eyssny10/sny10 standard conditions Days 30-44 retina
IHC Adult retina
IHC eyssny14/sny14 standard conditions Days 30-44 retina
IHC Adult retina
Ab2-gnat2 IHC WT control Days 30-44 retina
IHC Adult retina
IHC eyssny10/sny10 standard conditions Days 30-44 retina
IHC Adult retina
IHC eyssny14/sny14 standard conditions Days 30-44 retina
IHC Adult retina
Ab3-rho IHC WT control Days 30-44 retina
IHC Adult retina
IHC eyssny10/sny10 standard conditions Days 30-44 retina
IHC Adult retina
IHC eyssny14/sny14 standard conditions Days 30-44 retina
IHC Adult retina
Phenotype Details
Fish Conditions Stage Phenotype
eyssny10/sny10 standard conditions Adult retina ab2-gnat2 labeling decreased amount, abnormal
Adult retina ab1-opn1sw2 labeling decreased amount, abnormal
Adult retina ab3-rho labeling decreased amount, abnormal
Adult retina ab1-opn1sw1 labeling decreased amount, abnormal
Adult retina ab1-opn1mw1 labeling decreased amount, abnormal
Adult retinal cone cell decreased amount, abnormal
eyssny14/sny14 standard conditions Adult retina ab1-opn1sw2 labeling decreased amount, abnormal
Adult retina ab2-gnat2 labeling decreased amount, abnormal
Adult retina ab1-opn1sw1 labeling decreased amount, abnormal
Adult retina ab3-rho labeling decreased amount, abnormal
Adult retina ab1-opn1mw1 labeling decreased amount, abnormal
Adult retinal cone cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open