ZFIN ID: ZDB-FIG-161118-1
Tang et al., 2016 - Loss of a Candidate Biliary Atresia Susceptibility Gene, add3a, Causes Biliary Developmental Defects in Zebrafish. Journal of pediatric gastroenterology and nutrition   63(5):524-530 Full text @ J. Pediatr. Gastroenterol. Nutr.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 1

Expression of add3a and xpnpep1 in the developing liver. Whole-mount in situ hybridization (ISH) of 3 days post fertilization (dpf) zebrafish larvae probed for add1 (A), add2 (B), add3a (C), add3b (D), and xpnpep1 (E). There is liver staining for add3a (C, black arrow) and xpnpep1 (E, black arrow) but not for the other probes. There is intestinal staining for add3a, add3b and xpnpep1 (black arrowheads in C, D, E). There is also staining of add2 and add3a in the head region, which is of unclear specificity (B, C). Similar experiments were performed using a riboprobe directed against xpnpep2, but there was no staining in 3 dpf larvae (not shown).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
add1 TL standard conditions Protruding-mouth Not Detected liver ISH
add2 TL standard conditions Protruding-mouth head ISH
Protruding-mouth Not Detected liver ISH
add3a TL standard conditions Protruding-mouth head ISH
Protruding-mouth intestine ISH
Protruding-mouth liver ISH
add3b TL standard conditions Protruding-mouth intestine ISH
Protruding-mouth Not Detected liver ISH
xpnpep1 TL standard conditions Protruding-mouth intestine ISH
Protruding-mouth liver ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Journal of pediatric gastroenterology and nutrition for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Pediatr. Gastroenterol. Nutr.