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ZFIN ID: ZDB-FIG-160816-1
Venero Galanternik et al., 2015 - Heparan Sulfate Proteoglycans Regulate Fgf Signaling and Cell Polarity during Collective Cell Migration. Cell Reports   10(30):414-428 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 1

Loss of HSPGs Results in LL Prim Migration and Patterning Defects

(A, E, I, and M) gpc1b expression in (A) WT, (E) apcmcr, (I) DMSO-treated, and (M) SU5402-treated embryos.

(B, C, F, G, J, K, N, and O) gpc4 and sdc3 expression in (B and C) WT, (F and G) apcmcr, (J and K) DMSO-treated, and (N and O) SU5402-treated embryos.

(D, H, L, and P) sdc4 expression in (D) WT, (H) apcmcr, (L) DMSO-treated, and (P) SU5402-treated embryos.

(G and H) Black arrowheads point to the ectopic halo of sdc3 and sdc4.

(Q and R) WT and extl3 mutant prim has reached the tail tip by 48 hpf.

(S and T) ext2 and extl3/ext2 prim fails to complete migration.

(Q′-T′) Magnification of prim at 46 hpf.

(Q′ and R′) WT and extl3 mutant prim shows a normal morphology.

(S′) ext2 mutant prim possesses a pointed tip (white arrow).

(T′) extl3/ext2 mutant prim shows severe morphological defects. The leading edge elongates (white arrow) and rosettes are closely spaced (yellow arrowheads).

(U-W) Still images of time-lapse movies: WT (U), ext2 (V), and extl3/ext2 mutant prim (W). Yellow arrows in (V) and (W) point to ectopic filopodia.

See also Figure S2 and Movies S1, S2, S3, and S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP extl3tm70g/tm70g ; zf106Tg standard conditions High-pec posterior lateral line primordium IFL
Long-pec posterior lateral line primordium IFL
zf106Tg ; ext2to79c/to79c standard conditions High-pec posterior lateral line primordium IFL
Long-pec posterior lateral line primordium IFL
zf106Tg ; extl3tm70g/tm70g ; ext2to79c/to79c standard conditions High-pec posterior lateral line primordium IFL
Long-pec posterior lateral line primordium IFL
gpc1b WT standard conditions Prim-15 posterior lateral line primordium ISH
Prim-15 posterior lateral line primordium anatomical region ISH
WT chemical treatment by environment: SU5402 Prim-15 posterior lateral line primordium ISH
apchu745/hu745 standard conditions Prim-15 posterior lateral line primordium ISH
gpc4 WT standard conditions Prim-15 posterior lateral line primordium ISH
WT chemical treatment by environment: SU5402 Prim-15 posterior lateral line primordium ISH
apchu745/hu745 standard conditions Prim-15 posterior lateral line primordium ISH
sdc3 WT standard conditions Prim-15 posterior lateral line primordium ISH
WT chemical treatment by environment: SU5402 Prim-15 posterior lateral line primordium ISH
apchu745/hu745 standard conditions Prim-15 posterior lateral line primordium ISH
sdc4 WT standard conditions Prim-15 posterior lateral line primordium ISH
WT chemical treatment by environment: SU5402 Prim-15 Not Detected posterior lateral line primordium ISH
apchu745/hu745 standard conditions Prim-15 posterior lateral line primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: SU5402 Prim-15 posterior lateral line primordium sdc4 expression absent, abnormal
Prim-15 posterior lateral line primordium gpc4 expression decreased distribution, abnormal
Prim-15 posterior lateral line primordium gpc1b expression increased distribution, abnormal
Prim-15 posterior lateral line primordium sdc3 expression spatial pattern, abnormal
apchu745/hu745 standard conditions Prim-15 posterior lateral line primordium sdc4 expression decreased distribution, abnormal
Prim-15 posterior lateral line primordium sdc3 expression increased distribution, abnormal
Prim-15 posterior lateral line primordium gpc4 expression increased distribution, abnormal
Prim-15 posterior lateral line primordium gpc1b expression increased distribution, abnormal
Prim-15 posterior lateral line primordium sdc4 expression spatial pattern, abnormal
extl3tm70g/tm70g ; zf106Tg standard conditions High-pec posterior lateral line neuromast primordium migration process quality, normal
Long-pec posterior lateral line neuromast primordium migration process quality, normal
zf106Tg ; ext2to79c/to79c standard conditions High-pec neuromast decreased distance neuromast, abnormal
High-pec posterior lateral line neuromast deposition decreased rate, abnormal
High-pec posterior lateral line neuromast primordium migration arrested, abnormal
High-pec posterior lateral line primordium morphology, abnormal
Long-pec neuromast decreased distance neuromast, abnormal
Long-pec posterior lateral line neuromast deposition decreased rate, abnormal
Long-pec posterior lateral line neuromast primordium migration arrested, abnormal
Long-pec posterior lateral line primordium morphology, abnormal
zf106Tg ; extl3tm70g/tm70g ; ext2to79c/to79c standard conditions High-pec neuromast decreased distance neuromast, abnormal
High-pec posterior lateral line neuromast deposition decreased rate, abnormal
High-pec posterior lateral line neuromast primordium migration arrested, abnormal
High-pec posterior lateral line primordium increased size, abnormal
High-pec posterior lateral line primordium morphology, abnormal
Long-pec interneuromast cell has extra parts of type interneuromast cell cell projection, abnormal
Long-pec neuromast decreased distance neuromast, abnormal
Long-pec posterior lateral line neuromast has extra parts of type neuromast cell projection, abnormal
Long-pec posterior lateral line neuromast deposition decreased rate, abnormal
Long-pec posterior lateral line neuromast primordium migration arrested, abnormal
Long-pec posterior lateral line primordium increased size, abnormal
Long-pec posterior lateral line primordium morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.