ZFIN ID: ZDB-FIG-160525-14
Deshwar et al., 2016 - The Apelin receptor enhances Nodal/TGFβ signaling to ensure proper cardiac development. eLIFE   5 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 75%-epiboly
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: 30%-epiboly to Long-pec

Fig. 2

Aplnr deficient embryos exhibit a reduction in Nodal signaling.

(A) List and Venn diagram of 23 Nodal target genes found to be down-regulated in a microarray of aplnra/b morphant embryos compared to WT at 50% epiboly (5.25 hpf). (B) Relative luciferase activity regulated by the Activin response element (ARE) in WT, aplnra/b morpholino (MO), oep MO and lefty1/2 MO injected embryos at 30% epiboly (4.7 hpf). Data are represented as means ± SEM. *p<0.05 unpaired two-tailed t-test. (C-E) Phenotypic characterization of WT (D) and aplnra/b morphant embryos (E) when treated with the indicated concentration of the Alk4/5/7 inhibitor SB431542 from the sphere stage (4 hpf) onwards. (F-S) Visualization of the expression of the canonical nodal target genes gsc, flh and sox32 in WT (F,J,N), aplnra/b MOs injected (G,K,O), aplnrains; aplnrbhu4145 double mutant (H,L,P) and aplnrb RNA injected treated with the Aplnr agonist ML233 (I,M,Q) embryos at 8 hpf. Embryos are viewed from the dorsal side. Quantification of the number and spread of sox32 expressing cells (R,S). Data are represented as means ± SEM. *p<0.05, **p<0.01 unpaired two-tailed t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gsc WT standard conditions 75%-epiboly axial hypoblast ISH
WT + MO2-aplnrb + MO7-aplnra standard conditions 75%-epiboly axial hypoblast ISH
aplnrbhu4145/hu4145 ; aplnrazm00177433Tg/zm00177433Tg standard conditions 75%-epiboly axial hypoblast ISH
noto WT standard conditions 75%-epiboly axial hypoblast ISH
WT + MO2-aplnrb + MO7-aplnra standard conditions 75%-epiboly axial hypoblast ISH
aplnrbhu4145/hu4145 ; aplnrazm00177433Tg/zm00177433Tg standard conditions 75%-epiboly axial hypoblast ISH
sox32 WT standard conditions 75%-epiboly endodermal cell ISH
WT + MO2-aplnrb + MO7-aplnra standard conditions 75%-epiboly endodermal cell ISH
aplnrbhu4145/hu4145 ; aplnrazm00177433Tg/zm00177433Tg standard conditions 75%-epiboly endodermal cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: SB 431542 Long-pec eye fused with eye, abnormal
WT + MO1-tdgf1 standard conditions 30%-epiboly nodal signaling pathway decreased occurrence, abnormal
WT + MO2-aplnrb + MO7-aplnra standard conditions 30%-epiboly nodal signaling pathway decreased occurrence, abnormal
75%-epiboly axial hypoblast gsc expression decreased amount, abnormal
75%-epiboly axial hypoblast noto expression decreased amount, abnormal
75%-epiboly endodermal cell decreased amount, abnormal
75%-epiboly endodermal cell sox32 expression decreased amount, abnormal
75%-epiboly endodermal cell sox32 expression decreased distribution, abnormal
75%-epiboly nodal signaling pathway decreased occurrence, abnormal
WT + MO2-aplnrb + MO7-aplnra chemical treatment: SB 431542 Long-pec eye fused with eye, abnormal
WT + MO3-lft1 standard conditions 30%-epiboly nodal signaling pathway increased occurrence, abnormal
aplnrbhu4145/hu4145 ; aplnrazm00177433Tg/zm00177433Tg standard conditions 75%-epiboly axial hypoblast noto expression decreased amount, abnormal
75%-epiboly axial hypoblast gsc expression decreased amount, abnormal
75%-epiboly endodermal cell sox32 expression decreased amount, abnormal
75%-epiboly endodermal cell decreased amount, abnormal
75%-epiboly endodermal cell sox32 expression decreased distribution, abnormal
75%-epiboly nodal signaling pathway decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife