header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-160308-3
Shen et al., 2016 - The Rag-Ragulator Complex Regulates Lysosome Function and Phagocytic Flux in Microglia. Cell Reports   14(3):547-59 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 2

rraga Acts Autonomously at an Early Stage of Microglia Development

(A and B) Reduction of microglia in rragast77/st77 mutants is detected by imaging the mpeg1:EGFP transgene in living WT (A) and mutant (B) larvae at 5 dpf. Dorsal views, anterior to the top.

(C–H) Analysis of other microglia and macrophage markers reveals reduction of microglia at 3 dpf. Probes for apoe (C and D), slc7a7 (E and F), and mfap4 (G and H) were detected by whole-mount in situ hybridization. Boxes in (G) and (H) show region magnified in the corresponding insets. Lateral views, anterior to the left.

(I) Quantification of neutral red-stained microglia in rragast77/st77 mutants after transient expression of the WT rraga coding sequence under control of regulatory sequences from mpeg1, cldnk, and huc. Only mpeg1-rraga, which drives expression in macrophages and microglia, significantly rescued microglia in the mutants. Dotted lines at 15 and 25 show weak and strong rescues, respectively (****p ≤ 0.0001).

All scale bars, 50 µm. All larvae shown were genotyped by PCR after photography (A–H) or after visually scoring neutral red phenotypes (I).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
apoeb WT standard conditions Protruding-mouth brain microglial cell ISH
rragast77/st77 standard conditions Protruding-mouth brain microglial cell ISH
EGFP gl22Tg standard conditions Day 5 brain microglial cell IFL
gl22Tg ; rragast77/st77 standard conditions Day 5 brain microglial cell IFL
mfap4 WT standard conditions Protruding-mouth brain microglial cell ISH
rragast77/st77 standard conditions Protruding-mouth brain microglial cell ISH
slc7a7 WT standard conditions Protruding-mouth brain microglial cell ISH
rragast77/st77 standard conditions Protruding-mouth brain microglial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gl22Tg ; rragast77/st77 standard conditions Day 5 brain has fewer parts of type microglial cell, abnormal
Day 5 microglia development decreased process quality, abnormal
Day 5 microglial cell morphology, abnormal
rragast77/st77 standard conditions Protruding-mouth brain has fewer parts of type microglial cell, abnormal
rragast77/st77 standard conditions Protruding-mouth microglia development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.