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ZFIN ID: ZDB-FIG-160308-5
Shen et al., 2016 - The Rag-Ragulator Complex Regulates Lysosome Function and Phagocytic Flux in Microglia. Cell Reports   14(3):547-59 Full text @ Cell Rep.
Observed In:
Stage: Day 4

Fig. 4

Abnormal Vacuolar Organelles in Microglia and Uncleared Apoptotic Neurons in rragast77/st77 Mutants

(A–C) Transmission electron micrographs of the dorsal midbrain of (A) WT, (B and B′) rragast77/st77, and (C) irf8st95/st95 larvae at 4 dpf. Boxed region in (B) is shown at higher magnification in (B′). Microglia in the rragast77/st77 mutants have a striking accumulation of vacuolar organelles that appear to contain undigested material (white arrows). Microglia were absent in the irf8 mutant. In both mutants, uncleared corpses of apoptotic neurons are present (white arrowheads). These phenotypes also were evident in mutants stained with acridine orange ( Figure S3). Toluidene blue-stained sections of the same larvae are shown in Figure S4. Larvae were genotyped by PCR from tail biopsies collected immediately prior to fixation.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
irf8st95/st95 standard conditions Day 4 brain lacks all parts of type microglial cell, abnormal
Day 4 optic tectum apoptotic cell clearance decreased occurrence, abnormal
rragast77/st77 standard conditions Day 4 microglial cell lysosome organization process quality, abnormal
Day 4 microglial cell phagolysosome increased size, abnormal
Day 4 optic tectum apoptotic cell clearance decreased occurrence, abnormal
Day 4 optic tectum microglial cell has extra parts of type microglial cell phagolysosome, abnormal
Day 4 optic tectum microglial cell morphology, abnormal
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