ZFIN ID: ZDB-FIG-160225-26
Matsui et al., 2015 - A zebrafish jam-b2 Gal4-enhancer trap line recapitulates endogenous jam-b2 expression in extraocular muscles. Developmental dynamics : an official publication of the American Association of Anatomists   244(12):1574-80 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Day 4

Fig. 1

jam-b2 mRNA expression in zebrafish larvae. A1: jam-b2 is expressed ubiquitously at the 10 somite stage (scale bar = 200 µm). A2: High magnification image of A1 (scale bar = 100 µm). A3–A5: jam-b2 expression is identified in pectoral fins (arrow) and in cranial tissues (arrowhead) during 24–72 hr postfertilization (hpf; scale bar = 200 µm). A6: jam-b2 expression in EOMs and jaw muscles disappears at around 96 hpf (scale bar = 200 µm). B1: The signals detected in the cranial part represent EOMs and jaw muscles. Figures from the same embryo is presented. MF20: muscle specific antibody (scale bar = 100 µm). B2: jam-b2 expression at different focus along the dorsal–ventral axis. Figures are indicated from dorsal [D] to ventral [V] (scale bar = 200 µm). C1–C3: jam-b expression is identified in somites / myotomes, pectoral fins and cranial tissue during 24–72 hpf (scale bar = 200 µm). C4: The signals detected in the cranial part represent EOMs and jaw muscles. Figures from the same embryo is presented. MF20: muscle specific antibody (scale bar = 100 µm). D1–D2: jam-c expression is rather ubiquitous and does not show clear signals in muscles / myotomes compared with jam-b or jam-b2 (scale bar = 200 µm).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
jam2a AB control Prim-5 head ISH
Prim-5 pectoral fin bud ISH
Prim-5 somite ISH
Long-pec head ISH
Long-pec myotome ISH
Long-pec pectoral fin ISH
Protruding-mouth head ISH
Protruding-mouth myotome ISH
Protruding-mouth pectoral fin ISH
jam2b AB control 10-13 somites paraxial mesoderm ISH
Prim-5 extraocular musculature ISH
Prim-5 pectoral fin bud ISH
Prim-5 pharyngeal musculature ISH
Long-pec extraocular musculature ISH
Long-pec pectoral fin ISH
Long-pec pharyngeal musculature ISH
Protruding-mouth extraocular musculature ISH
Protruding-mouth pectoral fin ISH
Protruding-mouth pharyngeal musculature ISH
Day 4 cephalic musculature ISH
jam3b AB control Prim-5 whole organism ISH
Long-pec whole organism ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC AB control Protruding-mouth extraocular musculature
IHC Protruding-mouth pharyngeal musculature
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.