ZFIN ID: ZDB-PUB-150916-3
A zebrafish jam-b2 Gal4-enhancer trap line recapitulates endogenous jam-b2 expression in extraocular muscles
Matsui, H., Dorigo, A., Buchberger, A., Hocking, J.C., Distel, M., Köster, R.W.
Date: 2015
Source: Developmental dynamics : an official publication of the American Association of Anatomists   244(12): 1574-80 (Journal)
Registered Authors: Distel, Martin, Hocking, Jennifer, Köster, Reinhard W., Matsui, Hideaki
Keywords: Extraocular muscle, Junctional adhesion molecule, Zebrafish
MeSH Terms:
  • Animal Fins/embryology
  • Animal Fins/metabolism*
  • Animals
  • Animals, Genetically Modified
  • Gene Expression Regulation, Developmental
  • Junctional Adhesion Molecule B/genetics
  • Junctional Adhesion Molecule B/metabolism*
  • Muscle, Skeletal/embryology
  • Muscle, Skeletal/metabolism*
  • Zebrafish/embryology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 26370768 Full text @ Dev. Dyn.
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ABSTRACT
Members of the Junctional Adhesion Molecule (JAM) family function as cell adhesion molecules and cell surface receptors. The zebrafish genome contains six different jam genes, and jam-b and jam-c were shown to be essential for myoblast fusion during skeletal muscle development. However little is known about jam-b2 expression and function.
We isolated the cDNA of zebrafish jam-b2. jam-b2 is expressed specifically in EOMs, jaw muscles and pectoral fins in zebrafish larvae, but not in trunk muscles. The identified jam-b2 expression pattern is supported by the analysis of a zebrafish Gal4-enhancer trap line, in which the coding sequence of the transcriptional activator KalTA4 together with a Gal4-dependent UAS-mCherry expression cassette was inserted into the jam-b2 locus. Intercrosses with an UAS:EGFP strain proves the possibility for targeting transgene expression to EOMs, jaw muscles and fins. Finally, we characterized the concerted contraction pattern of EOMs in larvae performing an optokinetic response.
The expression pattern of jam-b2 suggests that it may contribute different properties to EOMs, jaw muscles and pectoral fins. The jam-b2:KalTA4-UAS-mCherry transgenic strain serves a dual role as both a reporter for these muscles and as a valuable genetic tool for targeting transgene expression to EOMs.
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