ZFIN ID: ZDB-FIG-151106-29
Ningappa et al., 2015 - The Role of ARF6 in Biliary Atresia. PLoS One   10:e0138381 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 2

arf6 knockdown results in developmental biliary defects in zebrafish.

(A, B) Whole-mount in situ hybridization showing arf6a and arf6b expression in developing embryos/larvae. Arrows point to the liver; arrowheads the pancreas; brackets mark the intestinal bulb. (C) The Tg(Tp1:GFP) and Tg(fabp10a:dsRed) lines reveal the intrahepatic biliary structure and liver size, respectively. Epifluorescence images showing the expression of these transgenes revealed a defect in the intrahepatic biliary structure in arf6-ATG MO-injected larvae. Based on the severity of the biliary defect, larvae were divided into three groups: normal, intermediate, and severe. Graph shows the percentage of larvae in each group. Arrows point to the liver; dotted lines outline the liver (A-C). (D) Epifluorescence images showing PED-6 accumulation in the gallbladder (arrows). Based on PED-6 levels in the gallbladder, larvae were divided into three groups: absent, small/faint, and normal. Graph shows the percentage of larvae in each group. n, the number of larvae examined; scale bars, 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
arf6a WT standard conditions Protruding-mouth intestinal bulb ISH
Protruding-mouth liver ISH
Protruding-mouth pancreas ISH
Day 4 liver ISH
arf6b WT standard conditions Protruding-mouth intestinal bulb ISH
Protruding-mouth liver ISH
Protruding-mouth pancreas ISH
Day 4 liver ISH
DsRed gz15Tg; um14Tg standard conditions Protruding-mouth liver IFL
gz15Tg; um14Tg + MO1-arf6a,arf6b standard conditions Protruding-mouth liver IFL
EGFP gz15Tg; um14Tg standard conditions Protruding-mouth intrahepatic bile duct IFL
gz15Tg; um14Tg + MO1-arf6a,arf6b standard conditions Protruding-mouth intrahepatic bile duct IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz15Tg; um14Tg + MO1-arf6a,arf6b standard conditions Protruding-mouth hepatocyte decreased functionality, abnormal
Protruding-mouth intrahepatic bile duct decreased amount, abnormal
Protruding-mouth liver decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One