ZFIN ID: ZDB-FIG-151106-36
Ningappa et al., 2015 - The Role of ARF6 in Biliary Atresia. PLoS One   10:e0138381 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. S5

Simultaneous knockdown of arf6a and arf6b results in more severe biliary defects than their single knockdown.

(A) The Tg(Tp1:GFP), Tg(fabp10a:dsRed), and Tg(ins:dsRed) lines were used to reveal the intrahepatic biliary structure, the liver, and the dorsal pancreas, respectively. Epifluorescence images showing the expression of these transgenes revealed that a severe biliary defect was observed more often in larvae co-injected with 3 ng of arf6a-UTR and 3 ng of arf6b-UTR MOs than in singly injected larvae. Based on the severity of the biliary defect, larvae were divided into three groups: normal, intermediate, and severe. Graph showing the percentage of larvae in each group. Arrows point to the liver; arrowheads point to the dorsal pancreas. Dotted lines outline the liver. Lateral views, anterior to the left. (B) Epifluorescence images showing PED-6 accumulation in the gallbladder. Based on PED-6 levels in the gallbladder, larvae were divided into three groups: absent, small/faint, and normal. Graph showing the percentage of larvae in each group. Arrows point to the gallbladder. Lateral views, anterior to the right. n indicates the number of larvae examined. Scale bars, 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed gz15Tg; um14Tg standard conditions Protruding-mouth liver IFL
gz15Tg; um14Tg + MO2-arf6a + MO2-arf6b standard conditions Protruding-mouth liver IFL
EGFP gz15Tg; um14Tg standard conditions Protruding-mouth intrahepatic bile duct IFL
gz15Tg; um14Tg + MO2-arf6a + MO2-arf6b standard conditions Protruding-mouth intrahepatic bile duct IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz15Tg; um14Tg + MO2-arf6a standard conditions Protruding-mouth intrahepatic bile duct decreased amount, abnormal
Protruding-mouth intrahepatic bile duct development disrupted, abnormal
Protruding-mouth liver decreased size, abnormal
gz15Tg; um14Tg + MO2-arf6a + MO2-arf6b standard conditions Protruding-mouth intrahepatic bile duct decreased amount, abnormal
Protruding-mouth intrahepatic bile duct development disrupted, abnormal
Protruding-mouth liver decreased size, abnormal
Day 5 hepatocyte decreased functionality, abnormal
gz15Tg; um14Tg + MO2-arf6b standard conditions Protruding-mouth intrahepatic bile duct decreased amount, abnormal
Protruding-mouth intrahepatic bile duct development disrupted, abnormal
Protruding-mouth liver decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One