ZFIN ID: ZDB-FIG-150512-1
Burgoyne et al., 2015 - Regulation of melanosome number, shape and movement in the zebrafish retinal pigment epithelium by OA1 and PMEL. Journal of Cell Science   128(7):1400-7 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 1

OA1 MOs reduce melanosome number at 2dpf without affecting melanosome size. (A–D) Contrast-enhanced images of zebrafish eye cross-sections highlighting only electron-dense melanin. Images are shown from (A,B) control and (C,D) OA1 MO-treated zebrafish. KD, knockdown. Scale bars: 20µm. (E) At 2dpf there is a significant reduction in melanin area between controls and OA1 MO-treated zebrafish. By 5dpf, the OA1 MO appears to be less effective, resulting in no significant difference in melanin area between controls and OA1 MO-treated animals. (F) The OA1 MO had no apparent effect on melanosome diameter at 2 and 5dpf. Results show the mean±s.e.m.; *P<0.05 (Student′s t-test).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gpr143 standard conditions Long-pec retinal pigmented epithelium decreased pigmentation, abnormal
Long-pec retinal pigmented epithelium melanosome decreased amount, abnormal
Long-pec retinal pigmented epithelium melanosome size, normal
Long-pec retinal pigmented epithelium melanosome spatial pattern, normal
Day 5 retinal pigmented epithelium melanosome amount, normal
Day 5 retinal pigmented epithelium melanosome size, normal
Day 5 retinal pigmented epithelium melanosome spatial pattern, normal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.