ZFIN ID: ZDB-FIG-150505-7
Lin et al., 2015 - Angiopoietin-like proteins stimulate HSPC development through interaction with Notch receptor signaling. eLIFE   4 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Prim-25

Fig. 4 NOTCH ChIP-seq in ANGPTL2-stimulated CD34+ cells.

(A) Enriched transcription factor motifs at the peaks of NOTCH binding sites from NOTCH ChIP-seq. Parentheses indicate E values for the significance of the motif. An embedded RBPjK ((consensus motif (red box) was found within the ZNF143 motif as previously described (Wang et al., 2011). (B) Examples of MYC-related categories from GREAT analysis of NOTCH ChIP-seq. (C) IPA network analysis of NOTCH ChIP-seq bound genes revealed a dense network of target genes centered on MYC.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myb WT control Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
WT + MO1-angptl1a + MO2-angptl2b control Prim-25 Not Detected ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
WT + MO1-mycb control Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
mib1ta52b/ta52b heat shock Prim-25 Not Detected ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-angptl1a + MO2-angptl2b control Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell myb expression absent, abnormal
Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell absent, abnormal
WT + MO1-mycb control Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell decreased amount, abnormal
WT + MO1-mycb control Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell myb expression decreased distribution, abnormal
mib1ta52b/ta52b heat shock Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell myb expression absent, abnormal
Prim-25 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife