ZFIN ID: ZDB-FIG-150402-29
Seredick et al., 2014 - Lhx3 and Lhx4 suppress Kolmer-Agduhr interneuron characteristics within zebrafish axial motoneurons. Development (Cambridge, England)   141(20):3900-9 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 7

Lhx3 and Lhx4 promote differentiation of late-born V2a and V2b INs. (A-D) Lateral views of 24hpf Tg(vsx1:GFP) embryos. (A,B,K) The number of early-born V2a INs labeled with vsx2 is unchanged in lhx3+lhx4 MO-injected embryos. (C,D,K) The number of early-born V2b INs labeled with tal1 is unchanged in lhx3+lhx4 MO-injected embryos. (E-H) Lateral views of 2dpf Tg(vsx1:GFP) embryos. (E,F) V2as labeled by GFP in 2dpf Tg(vsx2:EGFP) embryos are reduced in lhx3+lhx4 MO-injected embryos. (G,H) Single optical section; the number of V2bs co-labeled by gata3 and GFP in Tg(vsx1:GFP) embryos is reduced in lhx3+lhx4 MO-injected embryos. (I,J) Cross-section through mid-trunk of 2dpf Tg(vsx1:GFP) embryos. The number of V2s and late p2 progenitors labeled by GFP is unaffected in lhx3+lhx4 MO-injected embryos. Islet was included to exclude MNs that weakly express GFP (Reimer et al., 2013). (K) Quantification of early-born V2a and V2b INs (P>0.05; n=40 segments in ten embryos for each condition). (L) Quantification of V2a and V2b INs at 2dpf (PV2a=1×1011; PV2b=2×105; n=60 segments from ten embryos for each condition). (M) Quantification of V2 INs plus late p2 progenitors (P=0.14; n=10 alternating segments from ten embryos for each condition). In control embryos some weakly GFP+ cells co-express Islet; these cells are MNs and were not included in p2 progenitor and V2 IN counts. Scale bars: 20µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nns1Tg control Long-pec CiD IHC
nns1Tg + MO1-lhx3 + MO1-lhx4 standard conditions Long-pec CiD IHC
gata3 nns5Tg control Long-pec VeLD ISH
nns5Tg + MO1-lhx3 + MO1-lhx4 standard conditions Long-pec VeLD ISH
GFP nns5Tg control Prim-5 CiD IHC
Prim-5 VeLD IHC
Long-pec CiD IHC
Long-pec VeLD IHC
nns5Tg + MO1-lhx3 + MO1-lhx4 standard conditions Prim-5 CiD IHC
Prim-5 VeLD IHC
Long-pec CiD IHC
Long-pec VeLD IHC
tal1 nns5Tg control Prim-5 VeLD ISH
nns5Tg + MO1-lhx3 + MO1-lhx4 standard conditions Prim-5 VeLD ISH
vsx2 nns5Tg control Prim-5 CiD ISH
nns5Tg + MO1-lhx3 + MO1-lhx4 standard conditions Prim-5 CiD ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC nns5Tg control Long-pec spinal cord motor neuron
IHC nns5Tg + MO1-lhx3 + MO1-lhx4 standard conditions Long-pec spinal cord motor neuron
Phenotype Details
Fish Conditions Stage Phenotype
nns1Tg + MO1-lhx3 + MO1-lhx4 standard conditions Long-pec spinal cord has fewer parts of type CiD, abnormal
Long-pec ventral spinal cord interneuron differentiation decreased occurrence, abnormal
nns5Tg + MO1-lhx3 + MO1-lhx4 standard conditions Prim-5 spinal cord has normal numbers of parts of type CiD, normal
Prim-5 spinal cord has normal numbers of parts of type VeLD, normal
Prim-5 ventral spinal cord interneuron differentiation occurrence, normal
Long-pec spinal cord has fewer parts of type VeLD, abnormal
Long-pec ventral spinal cord interneuron differentiation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development