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ZIRC
ZFIN ID: ZDB-FIG-150313-25
De Smet et al., 2014 - Fibroblast Growth Factor Signaling Affects Vascular Outgrowth and Is Required for the Maintenance of Blood Vessel Integrity. Chemistry & Biology   21(10):1310-7 Full text @ Chem. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage Range: High-pec to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: High-pec to Protruding-mouth

Fig. 4

Role of FGFs in Other Angiogenesis Processes in Zebrafish

(A and B) Fluorescent images (ventral view) of GFP+ subintestinal vessels (SIVs) on the surface of the yolk sac of 72 hpf Tg(fli1:EGFP)y1 zebrafish embryos, revealing normal SIVs after exposure to DMSO (A) and severe underdevelopment of these SIVs after exposure to 50 μM SSR (tissue concentration 1.9 μg SSR/mg protein) from 20 hpf onward (arrowheads in B).

(C and D) Images (lateral view) of the GFP+ vascular plexus (VP) in the posterior trunk of 45 hpf Tg(fli1:EGFP)y1 zebrafish embryos. In DMSO treated control embryos (C), a vascular plexus formed via branching from the caudal vein. After exposure to 200 μM SSR (tissue concentration 3.9 μg SSR/mg protein) from 20 hpfs onward, the vascular plexus failed to remodel and branch and, instead, only a large vascular syncytium was formed (D). In all panels, the head of the embryo faces left; scale bars denote 50 μm in (A) and (B) and 100 μm in (C) and (D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control High-pec caudal vein plexus IFL
Protruding-mouth subintestinal vein IFL
y1Tg chemical treatment: pharmaceutical High-pec caudal vein plexus IFL
Protruding-mouth subintestinal vein IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg chemical treatment: pharmaceutical High-pec subintestinal vein morphology, abnormal
High-pec subintestinal vein blood vessel development process quality, abnormal
Protruding-mouth caudal vein plexus morphology, abnormal
Protruding-mouth caudal vein plexus blood vessel development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Chemistry & Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Chem. Biol.