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ZFIN ID: ZDB-FIG-150313-23
De Smet et al., 2014 - Fibroblast Growth Factor Signaling Affects Vascular Outgrowth and Is Required for the Maintenance of Blood Vessel Integrity. Chemistry & Biology   21(10):1310-7 Full text @ Chem. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to High-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-5 to High-pec

Fig. 2

FGFR Expression in Zebrafish Embryos

(A and B) Endothelial expression of FGFRs was determined by real-time RT-PCR analysis of GFP+ ECs isolated by flow cytometry from 24 hpf Tg(fli1:EGFP)y1 and Tg(flk1:EGFP) zebrafish embryos. Data are mean ± SEM.

(C-F) Whole-mount in situ hybridization of 24 hpf embryos revealed that fgfr1 and fgfr2 were expressed in the DA, PCV, intersegmental vessels (ISVs; arrows) and vascular plexus (VP) in the ventrocaudal tail, while expression of fgfr3 in these vessels was less pronounced. The inset in C and D displays a higher magnification of the fgfr1- and fgfr2-positive ISVs (red boxed ares). Fgfr3 and fgfr4 are both present in the vascular plexus (VP), while fgfr3 is faintly expressed in the DA, and fgfr4 is expressed in the caudal somites. Fgfr4 was mainly present in the vacular plexus at 24 hpf. Scale bars denote 100 ┬Ám in (C)-(F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Prim-5 posterior cardinal vein IFL
Prim-5 trunk vasculature IFL
High-pec dorsal aorta IFL
High-pec dorsal longitudinal anastomotic vessel IFL
High-pec intersegmental vessel IFL
High-pec posterior cardinal vein IFL
High-pec trunk vasculature IFL
y1Tg chemical treatment: pharmaceutical Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Prim-5 posterior cardinal vein IFL
Prim-5 trunk vasculature IFL
High-pec dorsal aorta IFL
High-pec dorsal longitudinal anastomotic vessel IFL
High-pec intersegmental vessel IFL
High-pec posterior cardinal vein IFL
High-pec trunk vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg chemical treatment: pharmaceutical Prim-5 intersegmental vessel collapsed, abnormal
High-pec dorsal longitudinal anastomotic vessel disassembled, abnormal
High-pec dorsal longitudinal anastomotic vessel endothelial cell detached from dorsal longitudinal anastomotic vessel endothelial cell, abnormal
High-pec intersegmental vessel disassembled, abnormal
High-pec intersegmental vessel endothelial cell detached from intersegmental vessel endothelial cell, abnormal
High-pec sprouting angiogenesis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Chemistry & Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Chem. Biol.