ZFIN ID: ZDB-FIG-150313-24
De Smet et al., 2014 - Fibroblast Growth Factor Signaling Affects Vascular Outgrowth and Is Required for the Maintenance of Blood Vessel Integrity. Chemistry & Biology   21(10):1310-7 Full text @ Chem. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 3

Inhibition of FGFRs Results in Reduced VE-Cadherin Junctions

Immunostaining of whole-mount zebrafish embryos for VE-cadherin (A and D, or red in C and F), showing intact and continuous EC-cell contacts in control-treated Tg(fli1:EGFP)y1 zebrafish embryos at 49 hpf (A–C), while SSR-treated zebrafish (exposure to 75 μM in the swimming water; tissue concentration 2.4 μg SSR/mg protein) (D–F) had a discontinuous VE-cadherin lining with reduced VE-cadherin-positive EC-cell juctions, explaining why ECs became disconnected from each other.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Long-pec intersegmental vessel IHC
Long-pec trunk vasculature IHC
y1Tg chemical treatment: pharmaceutical Long-pec intersegmental vessel IHC
Long-pec trunk vasculature IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-cdh5 IHC y1Tg control Long-pec blood vessel endothelial cell cell-cell junction
IHC y1Tg chemical treatment: pharmaceutical Long-pec blood vessel endothelial cell cell-cell junction
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg chemical treatment: pharmaceutical Long-pec blood vessel endothelial cell cell-cell junction decreased amount, abnormal
Long-pec intersegmental vessel endothelial cell decreased adhesivity, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Chemistry & Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Chem. Biol.