PUBLICATION

Fibroblast Growth Factor Signaling Affects Vascular Outgrowth and Is Required for the Maintenance of Blood Vessel Integrity

Authors
De Smet, F., Tembuyser, B., Lenard, A., Claes, F., Zhang, J., Michielsen, C., Van Schepdael, A., Herbert, J.M., Bono, F., Affolter, M., Dewerchin, M., Carmeliet, P.
ID
ZDB-PUB-140910-5
Date
2014
Source
Chemistry & Biology   21(10): 1310-7 (Journal)
Registered Authors
Affolter, Markus, Lenard, Anna
Keywords
none
MeSH Terms
  • Animal Fins/physiology
  • Animals
  • Blood Vessels/drug effects
  • Blood Vessels/metabolism*
  • Cadherins/metabolism
  • Embryo, Nonmammalian/metabolism
  • Indolizines/chemistry
  • Indolizines/metabolism
  • Indolizines/pharmacology
  • Intercellular Junctions/metabolism
  • Neovascularization, Physiologic/drug effects
  • Receptors, Fibroblast Growth Factor/antagonists & inhibitors
  • Receptors, Fibroblast Growth Factor/metabolism*
  • Regeneration
  • Signal Transduction/drug effects
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/metabolism*
  • ortho-Aminobenzoates/chemistry
  • ortho-Aminobenzoates/metabolism
  • ortho-Aminobenzoates/pharmacology
PubMed
25200605 Full text @ Chem. Biol.
Abstract
Angiogenesis contributes to the development of numerous disorders. Even though fibroblast growth factors (FGFs) were discovered as mediators of angiogenesis more than 30 years ago, their role in developmental angiogenesis still remains elusive. We use a recently described chemical probe, SSR128129E (SSR), that selectively inhibits the action of multiple FGF receptors (FGFRs), in combination with the zebrafish model to examine the role of FGF signaling in vascular development. We observe that while FGFR signaling is less important for vessel guidance, it affects vascular outgrowth and is especially required for the maintenance of blood vessel integrity by ensuring proper cell-cell junctions between endothelial cells. In conclusion, our work illustrates the power of a small molecule probe to reveal insights into blood vessel formation and stabilization and thus of broad interest to the vascular biology community.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes