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ZFIN ID: ZDB-FIG-150127-12
Hüsken et al., 2014 - Tcf7l2 Is Required for Left-Right Asymmetric Differentiation of Habenular Neurons. Current biology : CB   24(19):2217-27 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec

Fig. 3

Habenular Neurogenesis Is Not Overtly Affected in tcf7l2 Mutants

(A–E′, G, and G′) Dorsal views of the epithalamus, with anterior to the top.

(A and A′) The numbers and LR asymmetry of cxcr4b-expressing dHb neuronal precursors is not overtly affected in the tcf7l2 mutant.

(B–E′) HuC/D (B–C′) and transgenic HuC:GFP (D–E′) expression in differentiating habenular neurons in wild-type fry and tcf7l2 mutants. The dotted circles highlight the dHb.

(F) Numbers of HuC/D+ and HuC:GFP+ neurons are not significantly different between wild-type embryos and tcf7l2 mutants at 34, 36, 39, and 48 hpf. Error bars indicate the SEM.

(G and G′) BrdU (red)-labeled, 5 dpf dHb neurons expressing the pku588 transgene (green) in a wild-type (G) and a tcf7l2 mutant (G′).

(H and I) Graphs representing the number of BrdU-labeled cells (H) and the percentage of BrdU-labeled cells coexpressing the pku588 transgene (I) in wild-type embryos and tcf7l2 mutants. Error bars indicate the SD.

See also Table S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcr4b WT standard conditions Prim-15 dorsal habenular nucleus neuron ISH
tcf7l2u754/u754 standard conditions Prim-15 dorsal habenular nucleus neuron ISH
tcf7l2u763/u763 standard conditions Prim-15 dorsal habenular nucleus neuron ISH
EGFP knu3Tg standard conditions Prim-25 dorsal habenular nucleus neuron IHC
Long-pec dorsal habenular nucleus neuron IHC
pku588Tg standard conditions Prim-15 dorsal habenular nucleus IHC
tcf7l2u754/u754; knu3Tg standard conditions Prim-25 dorsal habenular nucleus neuron IHC
Long-pec dorsal habenular nucleus neuron IHC
tcf7l2u754/u754; pku588Tg standard conditions Prim-15 dorsal habenular nucleus IHC
tcf7l2u763/u763; knu3Tg standard conditions Prim-25 dorsal habenular nucleus neuron IHC
Long-pec dorsal habenular nucleus neuron IHC
tcf7l2u763/u763; pku588Tg standard conditions Prim-15 dorsal habenular nucleus IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT standard conditions Prim-15 dorsal habenular nucleus neuron
IHC Prim-25 dorsal habenular nucleus neuron
IHC tcf7l2u754/u754 standard conditions Prim-15 dorsal habenular nucleus neuron
IHC Prim-25 dorsal habenular nucleus neuron
IHC tcf7l2u763/u763 standard conditions Prim-15 dorsal habenular nucleus neuron
IHC Prim-25 dorsal habenular nucleus neuron
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.