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ZFIN ID: ZDB-FIG-150127-11
Hüsken et al., 2014 - Tcf7l2 Is Required for Left-Right Asymmetric Differentiation of Habenular Neurons. Current biology : CB   24(19):2217-27 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 2

tcf7l2 Mutants Show LR Symmetric Patterns of Epithalamic Neuron Differentiation

(A–F′) Dorsal views, with anterior to the top, of the habenulae of embryos labeled with markers at stages shown to the left. In (D–E′), nuclei (gray) are labeled with Topro3 and Sytox orange, respectively. All markers are symmetric in tcf7l2 mutants. Scale bars in (A)–(C′) represent 25 µm.

(G and G′) Dorsal views of 3D reconstructed confocal images of lipophilic-dye-labeled left (red) and right (green) habenular axons terminating in the IPN. The asterisk highlights the lack of axon terminals normally innervating the ventral IPN.

dHbl and dHbm, lateral and medial habenular subnucleus, respectively; dIPN and vIPN, dorsal and ventral interpeduncular nucleus, respectively; vHb, ventral habenular subnucleus. See also Figures S2 and S3 and Table S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cadps2 WT standard conditions Day 4 dorsal habenular nucleus ISH
tcf7l2u754/u754 standard conditions Day 4 dorsal habenular nucleus ISH
tcf7l2u763/u763 standard conditions Day 4 dorsal habenular nucleus ISH
cntn2 WT standard conditions Protruding-mouth dorsal habenular nucleus ISH
tcf7l2u754/u754 standard conditions Protruding-mouth Not Detected dorsal habenular nucleus ISH
tcf7l2u763/u763 standard conditions Protruding-mouth Not Detected dorsal habenular nucleus ISH
EGFP pku588Tg standard conditions Day 4 dorsal habenular nucleus IHC
tcf7l2u754/u754; pku588Tg standard conditions Day 4 dorsal habenular nucleus IHC
tcf7l2u763/u763; pku588Tg standard conditions Day 4 dorsal habenular nucleus IHC
GFP rw0110bTg standard conditions Day 4 dorsal habenular nucleus IHC
tcf7l2u754/u754; rw0110bTg standard conditions Day 4 dorsal habenular nucleus IHC
tcf7l2u763/u763; rw0110bTg standard conditions Day 4 dorsal habenular nucleus IHC
kctd12.2 WT standard conditions Day 4 habenula ISH
tcf7l2u754/u754 standard conditions Day 4 habenula ISH
tcf7l2u763/u763 standard conditions Day 4 habenula ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-SV2 IHC WT standard conditions Day 4 dorsal habenular nucleus
IHC Day 4 habenula
IHC Day 4 ventral habenular nucleus
IHC tcf7l2u754/u754 standard conditions Day 4 dorsal habenular nucleus
IHC Day 4 habenula
IHC Day 4 ventral habenular nucleus
IHC tcf7l2u763/u763 standard conditions Day 4 dorsal habenular nucleus
IHC Day 4 habenula
IHC Day 4 ventral habenular nucleus
Phenotype Details
Fish Conditions Stage Phenotype
tcf7l2u754/u754 standard conditions Day 4 habenula neuron projection displaced, abnormal
tcf7l2u763/u763 standard conditions Day 4 habenula neuron projection displaced, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.