ZFIN ID: ZDB-FIG-150127-10
Hüsken et al., 2014 - Tcf7l2 Is Required for Left-Right Asymmetric Differentiation of Habenular Neurons. Current biology : CB   24(19):2217-27 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 1

tcf7l2 Is Required for the Establishment of Habenular Asymmetry

(A and A′) Dorsal views of 4 dpf (days postfertilization) embryos, with anterior to the left, labeled with a cocktail of markers that reveal habenular asymmetry (kctd12.1) and position of liver (sepB) and pancreas (trypsin). The arrowheads indicate normal (A) and increased (A′) kctd12.1 expression in the right habenula.

(B and B′) Dorsal views of the habenulae with anterior to the top showing symmetric kctd12.1 expression in a wild-type fry (B) and a tcf7l2 mutant (B′).

(C) Diagram of intron/exon structure for tcf7l2 showing the tcf7l2exl [26], tcf7l2u763, and tcf7l2u754 alleles. Arrows point to mutation sites. The inset shows dorsal views, with anterior to the top, of 4 dpf wild-type (wt) and tcf7l2u754/u754 mutant embryos stained for Tcf7l2 (green) and Sytox orange (gray).

Hb, habenula; l, liver; p, pancreas. See also Figure S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kctd12.2 WT standard conditions Day 4 habenula ISH
tcf7l2u754/u754 standard conditions Day 4 habenula ISH
tcf7l2u763/u763 standard conditions Day 4 habenula ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-tcf7l2 IHC WT standard conditions Day 4 habenula
IHC tcf7l2u754/u754 standard conditions Day 4 Not Detected habenula
Phenotype Details
Fish Conditions Stage Phenotype
tcf7l2u754/u754 standard conditions Day 4 habenula determination of left/right asymmetry in diencephalon process quality, abnormal
tcf7l2u763/u763 standard conditions Day 4 habenula determination of left/right asymmetry in diencephalon process quality, abnormal
tcf7l2zf55/zf55 standard conditions Day 4 habenula determination of left/right asymmetry in diencephalon process quality, abnormal
Acknowledgments:
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