ZFIN ID: ZDB-FIG-150115-14
Huang et al., 2014 - Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development. Developmental Biology   394(1):83-93 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 5

PNC differentiation is regulated by endogenous RA signaling. (A and B) in situ hybridization for neuroD expression to detect 2° islets in 5 dpf pancreata from larvae transgenic for both the Notch-responsive creERT2driver, Tg(Tp1glob:creERT2)jh12, and the dnRAR cre responder, Tg(ubb:loxP-eCFP-loxP-dnRAR-GFP)jh39. These larvae had been incubated at 2 dpf with either vehicle (A) or 4OHT (B). (C) Average number of 2° islets/pancreas in vehicle or 4OHT treated larvae calculated by counting neuroD expressing islets. (D–G) Fluorescence immunostaining to detect hormone-expressing cells (insulin+/glucagons+/somatostatin+), facilitating quantification of 2° islet cells in larvae that were treated from 2–5 dpf with either vehicle (D), or 4OHT (E), or vehicle+DEAB (F). (E′′) enlarged image of (E′). (G) Average number of 2° islet cells/pancreas in vehicle, 4OHT, and vehicle+DEAB treated larvae calculated by counting for the number of hormone-expressing cells. **p<0.001. White dashes outline pancreata. 2° Islets in the tail region of the pancreas are indicated by arrowheads. N=number of larval pancreata quantified. Error Bar=SE. Scale Bar, 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
neurod1 jh12Tg; jh39Tg control Day 5 primary islet ISH
Day 5 secondary islet ISH
jh12Tg; jh39Tg chemical treatment: pharmaceutical Day 5 primary islet ISH
Day 5 secondary islet ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
jh12Tg; jh39Tg chemical treatment: pharmaceutical Day 5 pancreas has extra parts of type secondary islet, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 394(1), Huang, W., Wang, G., Delaspre, F., Vitery, M.D., Beer, R.L., Parsons, M.J., Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development, 83-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.