ZFIN ID: ZDB-FIG-150115-10 |
Gene: | |
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Fish: | |
Conditions: | |
Anatomical Terms: | |
Stage: | Day 5 |
Fish: | |
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Conditions: | |
Observed In: | |
Stage: | Day 5 |
Fig. 1 Notch and RA signaling regulate the induction of 2° islet cells in a manner consistent with synergy. (A–D) Confocal z-stack projections of larval Tg(neuroD:GFP)nl1 pancreata dissected at 5 dpf. Larvae were treated with (A) DMSO, (B) RO4929097 (RO) 0.625 μM, (C) DEAB 25 μM and (D) DEAB 25 μM+RO 0.625 μM, from 3–5 dpf. 2° Islet cells (green) in the tail region of pancreata are indicated by arrowheads. Pancreata are outlined in white dashed lines. Scale Bar, 100 μm. (E) Average number of 2° islet cells per 5 dpf larval pancreas. N=number of larval pancreata quantified. Error Bar represents standard error around the mean (SE). Significance, **p<0.001. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
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EGFP | nl1Tg | control | Day 5 | pancreas endocrine cell | IHC | |
Day 5 | primary islet | IHC | ||||
Day 5 | secondary islet | IHC | ||||
nl1Tg | chemical treatment: 4-(diethylamino)benzaldehyde | Day 5 | pancreas endocrine cell | IHC | ||
Day 5 | primary islet | IHC | ||||
Day 5 | secondary islet | IHC | ||||
nl1Tg | chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: pharmaceutical | Day 5 | pancreas endocrine cell | IHC | ||
Day 5 | primary islet | IHC | ||||
Day 5 | secondary islet | IHC | ||||
nl1Tg | chemical treatment: pharmaceutical | Day 5 | pancreas endocrine cell | IHC | ||
Day 5 | primary islet | IHC | ||||
Day 5 | secondary islet | IHC |
Acknowledgments: |
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Reprinted from Developmental Biology, 394(1), Huang, W., Wang, G., Delaspre, F., Vitery, M.D., Beer, R.L., Parsons, M.J., Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development, 83-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol. |
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