ZFIN Figure: Huang et al., 2014, Fig. 1

ZFIN ID: ZDB-FIG-150115-10

ZFIN ID: ZDB-FIG-150115-10

Huang et al., 2014 - Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development. Developmental Biology 394(1):83-93 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	EGFP
Fish:	nl1Tg
Conditions:	chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: pharmaceutical
Anatomical Terms:	pancreas endocrine cell primary islet secondary islet
Stage:	Day 5

PHENOTYPE:

Fish:	nl1Tg
Conditions:	chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: pharmaceutical
Observed In:	secondary islet cell
Stage:	Day 5

Fig. 1

Notch and RA signaling regulate the induction of 2° islet cells in a manner consistent with synergy. (A–D) Confocal z-stack projections of larval Tg(neuroD:GFP)^nl1 pancreata dissected at 5 dpf. Larvae were treated with (A) DMSO, (B) RO4929097 (RO) 0.625 μM, (C) DEAB 25 μM and (D) DEAB 25 μM+RO 0.625 μM, from 3–5 dpf. 2° Islet cells (green) in the tail region of pancreata are indicated by arrowheads. Pancreata are outlined in white dashed lines. Scale Bar, 100 μm. (E) Average number of 2° islet cells per 5 dpf larval pancreas. N=number of larval pancreata quantified. Error Bar represents standard error around the mean (SE). Significance, **p<0.001.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	nl1Tg	control	Day 5	pancreas endocrine cell	IHC
			Day 5	primary islet	IHC
			Day 5	secondary islet	IHC
	nl1Tg	chemical treatment: 4-(diethylamino)benzaldehyde	Day 5	pancreas endocrine cell	IHC
			Day 5	primary islet	IHC
			Day 5	secondary islet	IHC
	nl1Tg	chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: pharmaceutical	Day 5	pancreas endocrine cell	IHC
			Day 5	primary islet	IHC
			Day 5	secondary islet	IHC
	nl1Tg	chemical treatment: pharmaceutical	Day 5	pancreas endocrine cell	IHC
			Day 5	primary islet	IHC
			Day 5	secondary islet	IHC

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
nl1Tg	chemical treatment: 4-(diethylamino)benzaldehyde	Day 5	secondary islet cell increased amount, abnormal
nl1Tg	chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: pharmaceutical	Day 5	secondary islet cell increased amount, abnormal
nl1Tg	chemical treatment: pharmaceutical	Day 5	secondary islet cell increased amount, abnormal

Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from Developmental Biology, 394(1), Huang, W., Wang, G., Delaspre, F., Vitery, M.D., Beer, R.L., Parsons, M.J., Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development, 83-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 394(1), Huang, W., Wang, G., Delaspre, F., Vitery, M.D., Beer, R.L., Parsons, M.J., Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development, 83-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.