ZFIN ID: ZDB-FIG-150115-10
Huang et al., 2014 - Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development. Developmental Biology   394(1):83-93 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 5

Fig. 1

Notch and RA signaling regulate the induction of 2° islet cells in a manner consistent with synergy. (A–D) Confocal z-stack projections of larval Tg(neuroD:GFP)nl1 pancreata dissected at 5 dpf. Larvae were treated with (A) DMSO, (B) RO4929097 (RO) 0.625 μM, (C) DEAB 25 μM and (D) DEAB 25 μM+RO 0.625 μM, from 3–5 dpf. 2° Islet cells (green) in the tail region of pancreata are indicated by arrowheads. Pancreata are outlined in white dashed lines. Scale Bar, 100 μm. (E) Average number of 2° islet cells per 5 dpf larval pancreas. N=number of larval pancreata quantified. Error Bar represents standard error around the mean (SE). Significance, **p<0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nl1Tg control Day 5 pancreas endocrine cell IHC
Day 5 primary islet IHC
Day 5 secondary islet IHC
nl1Tg chemical treatment: 4-(diethylamino)benzaldehyde Day 5 pancreas endocrine cell IHC
Day 5 primary islet IHC
Day 5 secondary islet IHC
nl1Tg chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: pharmaceutical Day 5 pancreas endocrine cell IHC
Day 5 primary islet IHC
Day 5 secondary islet IHC
nl1Tg chemical treatment: pharmaceutical Day 5 pancreas endocrine cell IHC
Day 5 primary islet IHC
Day 5 secondary islet IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nl1Tg chemical treatment: 4-(diethylamino)benzaldehyde Day 5 secondary islet cell increased amount, abnormal
nl1Tg chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: pharmaceutical Day 5 secondary islet cell increased amount, abnormal
nl1Tg chemical treatment: pharmaceutical Day 5 secondary islet cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 394(1), Huang, W., Wang, G., Delaspre, F., Vitery, M.D., Beer, R.L., Parsons, M.J., Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development, 83-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.