|ZFIN ID: ZDB-IMAGE-150115-5|
PNC differentiation is regulated by endogenous RA signaling. (A and B) in situ hybridization for neuroD expression to detect 2° islets in 5 dpf pancreata from larvae transgenic for both the Notch-responsive creERT2driver, Tg(Tp1glob:creERT2)jh12, and the dnRAR cre responder, Tg(ubb:loxP-eCFP-loxP-dnRAR-GFP)jh39. These larvae had been incubated at 2 dpf with either vehicle (A) or 4OHT (B). (C) Average number of 2° islets/pancreas in vehicle or 4OHT treated larvae calculated by counting neuroD expressing islets. (D–G) Fluorescence immunostaining to detect hormone-expressing cells (insulin+/glucagons+/somatostatin+), facilitating quantification of 2° islet cells in larvae that were treated from 2–5 dpf with either vehicle (D), or 4OHT (E), or vehicle+DEAB (F). (E′′) enlarged image of (E′). (G) Average number of 2° islet cells/pancreas in vehicle, 4OHT, and vehicle+DEAB treated larvae calculated by counting for the number of hormone-expressing cells. **p<0.001. White dashes outline pancreata. 2° Islets in the tail region of the pancreas are indicated by arrowheads. N=number of larval pancreata quantified. Error Bar=SE. Scale Bar, 100 μm.
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