ZFIN ID: ZDB-FIG-150115-15
Huang et al., 2014 - Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development. Developmental Biology   394(1):83-93 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Fig. S1

Exogenous RA prevents the normal formation of secondary islets in larval fish. (A–B) Confocal z-stack projections of larval Tg(neuroD:GFP)nl1 pancreata dissected at 8 dpf. Larvae were treated with DMSO and different concentrations of RA from 5 to 8 dpf. 2° islet cells (green) in the tail region of pancreata are indicated by arrowheads. Pancreata are outlined in white dashed lines. Scale Bar, 100 μm. (C) Average number of 2° islet cells per 8 dpf larval pancreas. N=number of larval pancreata quantified. Error Bar represents standard error around the mean (SE). Significance, ***p<0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nl1Tg control Days 7-13 pancreas endocrine cell IHC
Days 7-13 primary islet IHC
Days 7-13 secondary islet IHC
nl1Tg chemical treatment: all-trans-retinoic acid Days 7-13 pancreas endocrine cell IHC
Days 7-13 primary islet IHC
Days 7-13 secondary islet IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nl1Tg chemical treatment: all-trans-retinoic acid Days 7-13 pancreas has fewer parts of type secondary islet, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 394(1), Huang, W., Wang, G., Delaspre, F., Vitery, M.D., Beer, R.L., Parsons, M.J., Retinoic acid plays an evolutionarily conserved and biphasic role in pancreas development, 83-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.