ZFIN ID: ZDB-FIG-140716-27
Duval et al., 2014 - gdf6a is required for cone photoreceptor subtype differentiation and for the actions of tbx2b in determining rod versus cone photoreceptor fate. PLoS One   9:e92991 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 6
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 4

Mutation in gdf6a does not disrupt tbx2b function in UV-versus-rod photoreceptor specification, but gdf6a rather plays a role in blue cone specification.

A,B. tbx2blor/lor mutants have fewer UV cones and more rods than wildtype fish (the lots-of-rods phenotype) (Kruskall-Wallis ANOVA, **p<0.005), but gdf6as327/s327 mutants have a normal abundance ratio and distribution of UV cones and rods (n = 10 wildtype, 8 tbx2blor/lor, and 7 gdf6as327/s327; UV cones expressing GFP and rods were labeled with antibody 4C12). Scale bars 30 µm and 80 µm, respectively. C. Larval gdf6as327/s327 mutants have a unique cone photoreceptor phenotype in which there are significantly fewer blue cones relative to UV cones at all ages examined (which is not observed in tbx2blor/lor or tbx2bfby/fby mutants- not shown) (Kruskall-Wallis ANOVA, ***p<0.001) Sample sizes at 3 days post-fertilization (dpf) are n = 17 larvae per genotype quantifying cells visualized via opsin in situ hybridization (Panel D); at 4 dpf data are from n = 9 wild type and n = 13 mutants assessed via GFP and mCherry transgene expression in cones; at 6dpf data are from 2 replicates of n = 4+7 wild type and n = 5+6 mutants assessed via transgene expression in cones (Panel E). D. UV and blue cones identified in 3 dpf by in situ hybridization against their respective opsins (Scale bars are 100 µm). E. UV and blue cones identified in transgenic lines at 6dpf by expression of GFP and mCherry, respectively (Scale bars are 60 μm and 40 μm in sibling and mutants, respectively).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP gdf6as327/s327; kj9Tg standard conditions Protruding-mouth to Day 6 UV sensitive photoreceptor cell IHC
gdf6as327/s327; kj9Tg; ua3011Tg standard conditions Day 6 UV sensitive photoreceptor cell IFL
kj9Tg standard conditions Protruding-mouth to Day 6 UV sensitive photoreceptor cell IHC
kj9Tg; ua3011Tg standard conditions Day 6 UV sensitive photoreceptor cell IFL
tbx2bp25bbtl/p25bbtl; kj9Tg standard conditions Protruding-mouth to Day 6 UV sensitive photoreceptor cell IHC
mCherry gdf6as327/s327; kj9Tg; ua3011Tg standard conditions Day 6 blue sensitive photoreceptor cell IFL
kj9Tg; ua3011Tg standard conditions Day 6 blue sensitive photoreceptor cell IFL
opn1sw1 WT standard conditions Protruding-mouth UV sensitive photoreceptor cell ISH
gdf6as327/s327 standard conditions Protruding-mouth UV sensitive photoreceptor cell ISH
opn1sw2 WT standard conditions Protruding-mouth blue sensitive photoreceptor cell ISH
gdf6as327/s327 standard conditions Protruding-mouth blue sensitive photoreceptor cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-4C12 IHC gdf6as327/s327; kj9Tg standard conditions Protruding-mouth - Day 6 retinal rod cell
IHC kj9Tg standard conditions Protruding-mouth - Day 6 retinal rod cell
IHC tbx2bp25bbtl/p25bbtl; kj9Tg standard conditions Protruding-mouth - Day 6 retinal rod cell
Phenotype Details
Fish Conditions Stage Phenotype
gdf6as327/s327 standard conditions Protruding-mouth retina has fewer parts of type blue sensitive photoreceptor cell, abnormal
gdf6as327/s327; kj9Tg; ua3011Tg standard conditions Day 4 retina has fewer parts of type blue sensitive photoreceptor cell, abnormal
Day 6 retina has fewer parts of type blue sensitive photoreceptor cell, abnormal
tbx2bp25bbtl/p25bbtl; kj9Tg standard conditions Protruding-mouth - Day 6 retina has extra parts of type retinal rod cell, abnormal
Protruding-mouth - Day 6 retina has fewer parts of type UV sensitive photoreceptor cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One