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ZIRC
ZFIN ID: ZDB-FIG-140716-28
Duval et al., 2014 - gdf6a is required for cone photoreceptor subtype differentiation and for the actions of tbx2b in determining rod versus cone photoreceptor fate. PLoS One   9:e92991 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 6

Fig. 5

A monoclonal antibody raised in rat (10C9.1) labels zebrafish UV cone outer segments, allowing all cone subtypes to be simultaneously labeled by immunohistochemistry.

Antibody 10C9.1 specifically labels the outer segments of a class of short single cones in the adult zebrafish retina (Figure S1 panel A). 10C9.1 specificity is supported (see Figure S1 panels B–D), including by a dramatic decrease in number of cells labeled when 10C9.1 is applied to retinas from zebrafish mutants (tbx2blor/lor) that have a paucity of UV cones. A. The population of single cones labeled by 10C9.1 is the UV cones, because established antibodies against the other single cone class, the blue cones, labels a distinct cone population (A2). 10C9.1 enables an unprecedented combination of antibodies raised in different species that simultaneously label and distinguish all cone photoreceptor subtypes (A′′). E. Further evidence that 10C9.1 labels UV cone outer segments comes from its co-localization with UV cones filled with green fluorescent protein (GFP), and its exclusion from blue cones filled with mCherry (mCh) in transgenic zebrafish (Tg(-5.5opn1sw1:EGFP)kj9;Tg(-3.5opn1sw2:m Cherry)ua3011). Panel B is available as Movie S1. Scale bars 30 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP kj9Tg; ua3011Tg standard conditions Protruding-mouth to Day 6 UV sensitive photoreceptor cell IHC
mCherry kj9Tg; ua3011Tg standard conditions Protruding-mouth to Day 6 blue sensitive photoreceptor cell IHC
opn1sw2 Ab1-opn1sw2 WT standard conditions Protruding-mouth to Day 6 blue sensitive photoreceptor cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-10C9.1 IHC WT standard conditions Protruding-mouth - Day 6 UV sensitive photoreceptor cell photoreceptor outer segment
IHC kj9Tg; ua3011Tg standard conditions Protruding-mouth - Day 6 UV sensitive photoreceptor cell photoreceptor outer segment
zpr-1 IHC WT standard conditions Protruding-mouth - Day 6 green sensitive photoreceptor cell
IHC Protruding-mouth - Day 6 red sensitive photoreceptor cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One