ZFIN ID: ZDB-FIG-140530-4
Blanco et al., 2014 - Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis. Disease models & mechanisms   7:547-59 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: ift88, ush1c
Antibodies: Ab1-cdh23, Ab1-ift88, Ab2-myo7a
Fish: WT, cdh23tj264/tj264, ift88tz288/tz288, myo7aaty220/ty220, ush1cfh293/fh293
Anatomical Term: hair cell
Stage: Day 5

Fig. 2 Cdh23 and Harmonin require Ift88 and Myo7aa for normal localization in hair cells. Immunolabeling in (A-D) wild-type siblings or (E-H) cdh23, (I-L) ush1c, (M-P) ift88 and (Q-T) myo7aa mutants of (A,E,I,M,Q) Cdh23, (B,F,J,N,R) Harmonin, (C,G,K,O,S) Ift88 and (D,H,L,P,T) Myo7aa. Confocal views of anterior macula hair cells are shown. Confocal sections are 0.8 µm thick, whereas the neck and basal region of the hair cell have an estimated diameter of 2-2.5 µm and 5-6 µm, respectively. Thus, not all confocal sections contain a hair bundle, and the observed shape of the hair bundle varies according to the mounting angle (see also supplementary material Fig. S3). Some individual hair cells are outlined by dotted lines. WT, wild type. Scale bar: 5 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ift88 Ab1-ift88 WT standard conditions Day 5 hair cell IHC
Ab1-ift88 cdh23tj264/tj264 standard conditions Day 5 hair cell IHC
Ab1-ift88 ift88tz288/tz288 standard conditions Day 5 Not Detected hair cell IHC
Ab1-ift88 myo7aaty220/ty220 standard conditions Day 5 hair cell IHC
Ab1-ift88 ush1cfh293/fh293 standard conditions Day 5 hair cell IHC
ush1c WT standard conditions Day 5 hair cell IHC
cdh23tj264/tj264 standard conditions Day 5 Not Detected hair cell IHC
ift88tz288/tz288 standard conditions Day 5 Not Detected hair cell IHC
myo7aaty220/ty220 standard conditions Day 5 hair cell IHC
ush1cfh293/fh293 standard conditions Day 5 Not Detected hair cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-cdh23 IHC WT standard conditions Day 5 hair cell
IHC cdh23tj264/tj264 standard conditions Day 5 Not Detected hair cell
IHC ift88tz288/tz288 standard conditions Day 5 Not Detected hair cell
IHC myo7aaty220/ty220 standard conditions Day 5 Not Detected hair cell
IHC ush1cfh293/fh293 standard conditions Day 5 Not Detected hair cell
Ab2-myo7a IHC WT standard conditions Day 5 hair cell
IHC cdh23tj264/tj264 standard conditions Day 5 hair cell
IHC ift88tz288/tz288 standard conditions Day 5 hair cell
IHC myo7aaty220/ty220 standard conditions Day 5 Not Detected hair cell
IHC ush1cfh293/fh293 standard conditions Day 5 hair cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.