FIGURE

Fig. 6

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ZDB-FIG-140311-11

Publication

Lahvic et al., 2013 - Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish

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Fig. 6

Concordance of asymmetry between heart and gut in hspb7 and hspb12 knockdown embryos. (A-D) Dorsal view, rostral toward bottom, left side as indicated in (A). Expression of foxa3 is strong in liver (Lv) and weaker in gut and pancreatic bud (P) at 48 hpf. (A′-D′) myl7 (cmlc2) is strongly expressed in ventricle (V) and more weakly in atrium (At) at 48 hpf. Anterior view, left side as indicated in (A). (A, A′) Control MO-injected embryo demonstrated the normal position of atrium to the left of the embryo′s midline and of the ventricle on the right (dextral loop). foxa3 is concentrated in the normal, left-sided liver and weaker in the pancreatic bud in control MO-injected embryo. (B, B′) hspb7 morphant with right-sided liver and ventricle. (C, C′) hspb12MO1-injected embryo with bilateral liver but normal position of the atrium and ventricle. (D, D′) hspb12MO1-injected embryo with left-sided liver and a heart that failed to jog or loop (midline heart).

Expression Data

Genes:	foxa3 myl7
Fish:	WT WT + MO3-hspb7 WT + MO3-hspb12
Knockdown Reagents:	MO3-hspb7 MO3-hspb12
Anatomical Terms:	atrium cardiac ventricle gut liver pancreatic bud
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-hspb7 WT + MO3-hspb12
Knockdown Reagents:	MO3-hspb7 MO3-hspb12
Observed In:	determination of digestive tract left/right asymmetry embryonic heart tube left/right pattern formation left/right pattern formation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 384(2), Lahvic, J.L., Ji, Y., Marin, P., Zuflacht, J.P., Springel, M.W., Wosen, J.E., Davis, L., Hutson, L.D., Amack, J.D., and Marvin, M.J., Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish, 166-180, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.