ZFIN ID: ZDB-FIG-140311-12
Lahvic et al., 2013 - Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish. Developmental Biology   384(2):166-180 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to 10-13 somites

Fig. 7

hspb7 knockdown disrupts cilia motility and asymmetric flow in Kupffer′s vesicle. (A) Analysis of cilia motility revealed that many cilia in hspb7 MO embryos were immotile or beat abnormally relative to controls. (B-C) Maximum projections of fluorescent bead movements superimposed on images of KV. Beads moved in a counterclockwise direction in control MO embryos (n=7/7 embryos; B), whereas this directional flow was absent in most hspb7 MO embryos (n=6/9 embryos; C). (D-F) Transmission electron micrographs showing: (D) transverse section of cilium from embryo injected with control MO showing 9+2 configuration of microtubules, (E) transverse section showing 9+0 configuration of microtubules in a cilium from embryo injected with hspb7 MO1+MO2, and (F) transverse section shows the abnormal central microtubule configuration in a cilium from embryo injected with hspb7 MO1. Arrows: outer dynein arms.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-hspb7 standard conditions 5-9 somites - 10-13 somites Kupffer's vesicle cilium movement process quality, abnormal
5-9 somites - 10-13 somites Kupffer's vesicle lacks parts or has fewer parts of type Kupffer's vesicle motile cilium, abnormal
5-9 somites - 10-13 somites Kupffer's vesicle motile cilium immobile, abnormal
10-13 somites Kupffer's vesicle motile cilium lacks parts or has fewer parts of type Kupffer's vesicle axonemal central pair, abnormal
10-13 somites Kupffer's vesicle motile cilium malformed, abnormal
WT + MO3-hspb7 + MO4-hspb7 standard conditions 10-13 somites Kupffer's vesicle motile cilium lacks parts or has fewer parts of type Kupffer's vesicle axonemal central pair, abnormal
10-13 somites Kupffer's vesicle motile cilium malformed, abnormal
WT + MO4-hspb7 standard conditions 5-9 somites - 10-13 somites Kupffer's vesicle cilium movement process quality, abnormal
5-9 somites - 10-13 somites Kupffer's vesicle lacks parts or has fewer parts of type Kupffer's vesicle motile cilium, abnormal
5-9 somites - 10-13 somites Kupffer's vesicle motile cilium immobile, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 384(2), Lahvic, J.L., Ji, Y., Marin, P., Zuflacht, J.P., Springel, M.W., Wosen, J.E., Davis, L., Hutson, L.D., Amack, J.D., and Marvin, M.J., Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish, 166-180, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.