ZFIN ID: ZDB-FIG-140311-7
Lahvic et al., 2013 - Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish. Developmental Biology   384(2):166-180 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2

Heart jog direction was randomized by hspb7 knockdown. (A) Two splice-blocking morpholinos that target hspb7 both significantly increased right and midline heart jogs at the expense of leftward heart jogs (Χ2 test, p<0.001). (B, C) In situ hybridization of myl7 (cmlc2) in embryos injected with hspb7MO1. (B) Left jog and (C) right jog. (D) Live embryo at 26 hpf, injected with control MO. (E) Live embryo at 26 hpf, injected with hspb7MO1, showing characteristic downward curl of the body. (F) The combination of hspb7MO1 (0.25 pmole) and hspb7MO2 (0.17 pmole) prevented heart field fusion when targeted to the whole embryo at 1-4 cell stages. (G-I) In situ hybridization for myl7 (cmlc2). (G) Embryo injected with control MO at 1 cell stage had normal leftward heart jogs; the anterior boundary of the heart is midway across the eye at 26 hpf. (H) Midline heart in embryo injected with hspb7MO1+MO2 at 1 cell stage, showing slowed anterior migration, with the anterior boundary of the heart at the posterior end of the eye. (I) hspb7MO1+MO2 injected embryo, with normal leftward jog but delayed anterior migration and wide heart. (J) hspb7MO1+MO2 injected embryo, with cardia bifida. (K) Yolk injection between 3 and 4 hpf of control MO produced a small number of midline hearts; hspb7MO1 (0.5 pmole) induced midline hearts; hspb7MO1 (0.25 pmole) and hspb7MO2 (0.25 pmole) induced cardia bifida and midline hearts.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 WT control Prim-5 primitive heart tube ISH
WT + MO3-hspb7 standard conditions Prim-5 primitive heart tube ISH
WT + MO3-hspb7 + MO4-hspb7 standard conditions Prim-5 primitive heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-hspb7 standard conditions Prim-5 heart jogging process quality, abnormal
Prim-5 heart rudiment displaced to whole organism axis, abnormal
Prim-5 heart rudiment displaced to whole organism right side, abnormal
Prim-5 heart rudiment malformed, abnormal
Prim-5 whole organism curved ventral, abnormal
WT + MO3-hspb7 + MO4-hspb7 standard conditions Prim-5 heart jogging process quality, abnormal
Prim-5 heart rudiment displaced to whole organism axis, abnormal
Prim-5 heart rudiment displaced to whole organism right side, abnormal
Prim-5 heart rudiment malformed, abnormal
Prim-5 heart rudiment split bilaterally, abnormal
WT + MO4-hspb7 standard conditions Prim-5 heart jogging process quality, abnormal
Prim-5 heart rudiment displaced to whole organism axis, abnormal
Prim-5 heart rudiment displaced to whole organism right side, abnormal
Prim-5 heart rudiment malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 384(2), Lahvic, J.L., Ji, Y., Marin, P., Zuflacht, J.P., Springel, M.W., Wosen, J.E., Davis, L., Hutson, L.D., Amack, J.D., and Marvin, M.J., Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish, 166-180, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.