ZFIN ID: ZDB-FIG-140117-18
Nagashima et al., 2013 - A self-renewing division of zebrafish Muller glial cells generates neuronal progenitors that require N-cadherin to regenerate retinal neurons. Development (Cambridge, England)   140(22):4510-4521 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 2

Müller glia partially dedifferentiate and express retinal progenitor markers in response to photoreceptor lesions. (A-D) PCNA (green), BLBP (cyan) and Rx1 (white/magenta) in control (ctrl) retina. Higher magnifications of the boxed regions are shown on the right. (A) Cones are Rx1+ (asterisks, UV cone nuclei). (B) By 1 hpl, Rx1 is reduced in UV cones and BLBP is upregulated in Müller glia. (C) At 8 hpl, cones collapse (asterisk) and Müller glia express BLBP and Rx1 (boxed region). A PCNA+ microglia or rod precursor (arrowhead) can be seen in the ONL. (D) At 1 dpl, Müller glia are weakly PCNA+/BLBP+/Rx1+. (E,F) Injury-induced nGFP in lesioned area (asterisks) at 4 and 8 hpl and in immature Müller glia (arrowheads). Zpr1 (magenta), which recognizes Arrestin 3a and specifically labels red/green cones. Higher magnifications of the boxed regions are shown in insets. (C) Reduced rlbp1a transcript (white) by 4 hpl and no detectable signal at 1 dpl. Scale bars: 10 μm (A-D,G); 100 μm (E,F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arr3a zpr-1 mi2004Tg control Adult retinal cone cell IHC
zpr-1 mi2004Tg light ablation: photoreceptor cell Adult retinal cone cell IHC
EGFP mi2004Tg control Adult Not Detected Muller cell nucleus IHC
mi2004Tg light ablation: photoreceptor cell Adult Muller cell nucleus IHC
rlbp1a WT standard conditions Adult Muller cell ISH
WT light ablation: photoreceptor cell Adult Not Detected Muller cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-rx1 IHC WT standard conditions Adult Muller cell
IHC Adult retinal cone cell
IHC WT light ablation: photoreceptor cell Adult Muller cell
Ab3-fabp7 IHC WT standard conditions Adult Muller cell
IHC WT light ablation: photoreceptor cell Adult Muller cell
Phenotype Details
Fish Conditions Stage Phenotype
WT light ablation: photoreceptor cell Adult Muller cell proliferative, abnormal
Adult retina has fewer parts of type eye photoreceptor cell, abnormal
Adult retina sensory epithelium regeneration increased occurrence, abnormal
mi2004Tg light ablation: photoreceptor cell Adult Muller cell proliferative, abnormal
Adult retina has fewer parts of type eye photoreceptor cell, abnormal
Adult retina sensory epithelium regeneration increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development