ZFIN ID: ZDB-FIG-140107-59
Weijts et al., 2013 - Atypical E2fs Control Lymphangiogenesis through Transcriptional Regulation of Ccbe1 and Flt4. PLoS One   8(9):e73693 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 3

Figure 3. E2f7/8 rescued Ccbe1dependent lymphangiogenesis phenotype.

A, Representative images of Tg(fli1a:gfp;flt1enh:rfp) un-injected control embryos (nic) or embryos injected with e2f7/8 MOs or mRNA. B, C, D, Quantification of the indicated parameters. Concentrations: e2f7/8 MOs (10 ng each); ccb1 MO (5 ng); e2f7/8 mRNA (100 pg each); ccbe1 mRNA (100 pg).Open arrow heads indicate in (A; upper panel) missing dorsal longitudinal anastomotic vessels. Closed arrow heads indicate (upper panel in A) PLs or (lower panel, A) presence of the TD. All scale bars are 100 μm. Stars indicate missing TD fragments. Data presented as the average (±s.e.m.) compared to the control condition in three independent experiments (*** P<0.001). At least n = 150 embryos per condition in three independent experiments were used for A–D.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hu5333Tg; y1Tg + MO1-ccbe1 standard conditions Long-pec lymphangioblast cord lymphangioblast decreased amount, abnormal
Day 5 thoracic duct morphology, abnormal
hu5333Tg; y1Tg + MO1-ccbe1 + MO1-e2f7 + MO1-e2f8 standard conditions Long-pec dorsal longitudinal anastomotic vessel separated from dorsal longitudinal anastomotic vessel, abnormal
Long-pec dorsal longitudinal anastomotic vessel sprouting angiogenesis disrupted, abnormal
Long-pec lymphangioblast cord lymphangioblast decreased amount, abnormal
Long-pec lymphangiogenesis disrupted, abnormal
Day 5 thoracic duct morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One