ZFIN ID: ZDB-FIG-140107-60
Weijts et al., 2013 - Atypical E2fs Control Lymphangiogenesis through Transcriptional Regulation of Ccbe1 and Flt4. PLoS One   8(9):e73693 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 4

E2f7/8 rescued Flt4-dependent lymphangiogenesis phenotypes.

A, Representative images of Tg(fli1a:gfp;flt1enh:rfp) un-injected control embryos (nic) or embryos injected with e2f7/8 MOs or mRNA. B, C, D, E Quantification of the indicated parameters. Concentrations: e2f7/8 MOs (10 ng each); dll4 MO (3 ng); e2f7/8 mRNA (100 pg each); dll4 mRNA (100 pg).Open arrow heads indicate in (A; upper panel) hyper-branching of intersegmental vessels. Closed arrow heads indicate (upper panel in A) PLs or (lower panel, A) presence of the TD. All scale bars are 100 µm. Stars indicate missing TD fragments. Data presented as the average (±s.e.m.) compared to the control condition in three independent experiments (*** P<0.001). At least n = 150 embryos per condition in three independent experiments were used for A–E.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hu5333Tg; y1Tg + MO1-e2f7 + MO1-e2f8 + MO4-dll4 standard conditions Long-pec intersegmental artery decreased amount, abnormal
Long-pec intersegmental vessel increased branchiness, abnormal
Long-pec lymphangioblast cord lymphangioblast decreased amount, abnormal
Long-pec lymphangiogenesis disrupted, abnormal
Long-pec sprouting angiogenesis disrupted, abnormal
Day 5 intersegmental artery decreased amount, abnormal
Day 5 intersegmental vessel increased branchiness, abnormal
Day 5 lymphangioblast decreased amount, abnormal
Day 5 lymphangiogenesis disrupted, abnormal
Day 5 sprouting angiogenesis disrupted, abnormal
Day 5 thoracic duct absent, abnormal
hu5333Tg; y1Tg + MO4-dll4 standard conditions Long-pec intersegmental artery decreased amount, abnormal
Long-pec intersegmental vessel increased branchiness, abnormal
Long-pec lymphangioblast cord lymphangioblast decreased amount, abnormal
Long-pec lymphangiogenesis disrupted, abnormal
Long-pec sprouting angiogenesis disrupted, abnormal
Day 5 intersegmental artery decreased amount, abnormal
Day 5 intersegmental vessel increased branchiness, abnormal
Day 5 lymphangioblast decreased amount, abnormal
Day 5 lymphangiogenesis disrupted, abnormal
Day 5 sprouting angiogenesis disrupted, abnormal
Day 5 thoracic duct absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One