ZFIN ID: ZDB-FIG-140107-58
Weijts et al., 2013 - Atypical E2fs Control Lymphangiogenesis through Transcriptional Regulation of Ccbe1 and Flt4. PLoS One   8(9):e73693 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 2 Loss of E2f7/8 impaired venous sprouting and lymphangiogenesis.

A, In situ hybridisation and B, qPCR (** P<0.05; two independent experiments with n = 10 per condition and experiment) for flt4 and ccbe1 in zebrafish embryos 32 hpf, un-injected control (nic) or injected with e2f7/8 MOs or mRNA. C, Flt4:YFP transgene level of 36 hpf uninjected or e2f7/8 MOs injected embryos, lateral view (n = 30 per condition). D–G Lateral images and quantification of Tg(fli1a:gfp;flt1enh:rfp) embryos treated as indicated and imaged at 52 hpf or 5 dpf. H–J Lateral images and quantification of Tg(fli1a:gfp;flt1enh:rfp) embryos treated as indicated and imaged at 52 hpf or 5 dpf. Concentrations: e2f7/8 MOs (10 ng each); e2f7/8 mRNA (100 pg each); ccbe1 mRNA (100 pg). Open arrow heads indicate missing intersegmental vessels or dorsal longitudinal anastomotic vessel. Closed arrow heads indicate PLs (upper panel) or the TD (lower panel). Arrows depict PLs that have connected to ISVs. Stars indicate missing TD fragments. All scale bars are 100 μm. Data presented as the average (±s.e.m.) compared to the control condition in three independent experiments (*** P<0.001). At least n = 150 embryos per condition in three independent experiments were used for D–J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccbe1 WT control Prim-15 axial vasculature ISH
WT + MO1-e2f7 + MO1-e2f8 standard conditions Prim-15 axial vasculature ISH
flt4 WT control Prim-15 axial vasculature ISH
WT + MO1-e2f7 + MO1-e2f8 standard conditions Prim-15 axial vasculature ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hu5333Tg; y1Tg + MO1-e2f7 + MO1-e2f8 standard conditions Long-pec intersegmental artery decreased amount, abnormal
Long-pec intersegmental vein angiogenic sprout decreased amount, abnormal
Long-pec intersegmental vessel decreased amount, abnormal
Long-pec lymphangioblast cord lymphangioblast decreased amount, abnormal
Day 5 intersegmental vein decreased amount, abnormal
Day 5 thoracic duct lymphangiogenesis disrupted, abnormal
Day 5 thoracic duct morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One