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ZFIN ID: ZDB-FIG-130815-8
Gupta et al., 2013 - Loss of Catalytically Inactive Lipid Phosphatase Myotubularin-related Protein 12 Impairs Myotubularin Stability and Promotes Centronuclear Myopathy in Zebrafish. PLoS Genetics   9(6):e1003583 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 4

Ultrastructural abnormalities in MTMR12 deficiency.

Transmission electron micrographs of skeletal muscles in control and morphant fish. In comparison to wild-type controls (A), mtmr12 morphant fish displayed abnormal triads (C, high magnification, inset). mtm1 morphant muscle also had absent or disorganized triads (B). mtmr12-mtm1 double knockdown morphants displayed exacerbated defects in muscle with many absent Z-lines in addition to disorganized triads (D inset shows high magnification view). Higher magnification examination showed whorled membranous structures in mtmr12 and mtmr12-mtm1 morphants (E–F). (G) Histograms represent quantification of disorganized triads of the single or double morphants. Total number of triads were counted in at least 15 myofibers within each embryo (n = 5 embryos). *Pd0.05, ns: statistically not significant.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-mtmr12 standard conditions Protruding-mouth skeletal muscle T-tubule absent, abnormal
Protruding-mouth skeletal muscle T-tubule disorganized, abnormal
AB + MO2-mtmr12 + MO4-mtm1 standard conditions Protruding-mouth skeletal muscle non-functional, abnormal
Protruding-mouth skeletal muscle Z disc absent, abnormal
AB + MO4-mtm1 standard conditions Protruding-mouth skeletal muscle T-tubule absent, abnormal
Protruding-mouth skeletal muscle T-tubule disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.