header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-130815-7
Gupta et al., 2013 - Loss of Catalytically Inactive Lipid Phosphatase Myotubularin-related Protein 12 Impairs Myotubularin Stability and Promotes Centronuclear Myopathy in Zebrafish. PLoS Genetics   9(6):e1003583 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 3

Abnormal histology of MTMR12-deficient zebrafish.

(A)Toluidine blue stained longitudinal sections of skeletal muscle in control and morphant fish at 3 dpf. In comparison to the control fish, mtmr12 morphants showed disorganized myofibers (arrowhead) with central nucleation (arrow), similar to histological changes observed in the skeletal muscle of mtm1 morphant fish (arrow). Knockdown of both mtm1 as well as mtmr12 results in severe muscle disorganization greater than seen in mtm1 or mtmr12 alone morphants. (B) Centrally nucleated myofibers were quantified. Serial sections from 3–4 different embryos were analyzed and the relative number of centrally nucleated fibers in the middle somites (10–13) were counted. (C) Hematoxylin and Eosin staining of mtmr12 morphant zebrafish at different time points. An increase in sarcomeric disorganization was observed at 3 dpf in comparison to 2 dpf in mtmr12 morphants (arrow) (D) Centrally nucleated myofibers were quantified. Serial sections from 6 different embryos were analyzed and the relative number of centrally nucleated fibers in the middle somites (10–13) were counted. Scale bar = 10 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-mtmr12 standard conditions Long-pec muscle cell nucleus displaced to muscle cell central region, abnormal
Long-pec skeletal muscle sarcomere disorganized, abnormal
Protruding-mouth muscle cell nucleus displaced to muscle cell central region, abnormal
Protruding-mouth skeletal muscle myofibril disorganized, abnormal
Protruding-mouth skeletal muscle sarcomere disorganized, abnormal
AB + MO2-mtmr12 + MO4-mtm1 standard conditions Protruding-mouth muscle cell nucleus displaced to muscle cell central region, abnormal
Protruding-mouth skeletal muscle myofibril disorganized, abnormal
Protruding-mouth skeletal muscle sarcomere disorganized, abnormal
AB + MO4-mtm1 standard conditions Protruding-mouth muscle cell nucleus displaced to muscle cell central region, abnormal
Protruding-mouth skeletal muscle myofibril disorganized, abnormal
Protruding-mouth skeletal muscle sarcomere disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.