ZFIN ID: ZDB-FIG-130815-9
Gupta et al., 2013 - Loss of Catalytically Inactive Lipid Phosphatase Myotubularin-related Protein 12 Impairs Myotubularin Stability and Promotes Centronuclear Myopathy in Zebrafish. PLoS Genetics   9(6):e1003583 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth

Fig. 5

Myotubularin and PtdIns3P alterations in mtmr12 morphants.

(A) Immunofluorescence of control and mtmr12 knockdown fish showed significantly decreased myotubularin staining in mtmr12 knockdown fish in images taken under identical conditions. Immunofluorescence detection of PtdIns3P showed apparent increases of this myotubularin substrate in mtmr12 morphant embryos as compared to controls. (B) PtdIns3P levels are increased in mtmr12, mtm1 and mtm1-mtmr12 morphant zebrafish, *Pd0.05. Total lipids were extracted from zebrafish at 3 dpf and PtdIns3P levels were measured using a lipid-protein overlay enzyme-linked immunosorbent assay.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mtm1 IHC AB control Protruding-mouth skeletal muscle T-tubule
IHC AB + MO2-mtmr12 standard conditions Protruding-mouth skeletal muscle T-tubule
Ab1-ptdIns(3,4,5)P3 IHC AB control Protruding-mouth skeletal muscle T-tubule
IHC AB + MO2-mtmr12 standard conditions Protruding-mouth skeletal muscle T-tubule
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.