ZFIN ID: ZDB-FIG-130815-32
Nishiwaki et al., 2013 - The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects. Developmental Cell   25(4):374-387 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3 Photoreceptor Degeneration in the coa Mutant Depends on BNip1 (A) Zpr1 labeling (green) of 84 hpf WT, coa mutants, coa mutants injected with egfp mRNA, and bcl2 mRNA. Arrowheads indicate rescued photoreceptors. (B) Percentage of zpr1-positive areas relative to the total retinal area in the experiments shown in (A). Green and black bars indicate the mean and SD, respectively. **p < 0.01. (C) Zpr1 labeling (green) of 84 hpf coa mutant and coa mutants injected with MO-Bax1, MO-Bax2, or a mixture of MO-Bax1 and MO-Bax2. Arrowheads indicate rescued photoreceptors. (D) Percentage of zpr1-positive areas relative to the total retinal area in the experiments shown in (C) as well as in a coa mutant injected with the standard MO. Green and black bars indicate the mean and SD, respectively. **p < 0.01 and *p < 0.05. (E) TUNEL staining (green) of 84 hpf WT, coa mutant, coa mutant retinas injected with misMO-BNip1a/b, and a mixture of MO-BNip1a and MO-BNip1b. Arrowheads indicate apoptosis in the ONL. (F) Percentage of TUNEL-positive areas relative to the total retinal area in the experiments shown in (E). Green and black bars indicate the mean and SD, respectively. **p < 0.01. (G) Zpr1 labeling (green) and GFP-tagged peripherin (magenta) in 84 hpf WT, coa mutant, coa mutant retinas injected with misMO-BNip1a/b or a mixture of MO-BNip1a and MO-BNip1b. Arrowheads indicate rescued photoreceptors. Nonspecific zpr1 signal was observed in the lens. (H) Percentage of zpr1-positive areas relative to the total retinal area in the experiments shown in (G). Green and black bars indicate the mean and SD, respectively. **p < 0.01. (I) Zpr1 labeling (green) and GFP-tagged peripherin or rhodopsin (magenta) in the photoreceptor cell layer in WT and coa mutants injected with MO-BNip1a and MO-BNip1b. Lower panels show the magenta channel. Arrowheads indicate the failure of transport to the OS.The numbers of retinal sections used in the experiments shown in (B), (D), (F), and (H) and p values for the t test are shown in Table S2. Scale bars, 50 μm (A, C, E, and G) and 10 μm (I). See also Figures S3 and S4.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC WT standard conditions Protruding-mouth retinal cone cell
IHC napbbrw76b/rw76b standard conditions Protruding-mouth retinal cone cell
IHC napbbrw76b/rw76b + MO1-baxb standard conditions Protruding-mouth retinal cone cell
IHC napbbrw76b/rw76b + MO1-baxb + MO2-baxa standard conditions Protruding-mouth retinal cone cell
IHC napbbrw76b/rw76b + MO1-bnip1a + MO1-bnip1b standard conditions Protruding-mouth retinal cone cell
IHC napbbrw76b/rw76b + MO2-baxa standard conditions Protruding-mouth retinal cone cell
Phenotype Details
Fish Conditions Stage Phenotype
napbbrw76b/rw76b standard conditions Protruding-mouth photoreceptor cell apoptotic, abnormal
Protruding-mouth retina apoptotic process increased occurrence, abnormal
Protruding-mouth retinal cone cell decreased amount, abnormal
napbbrw76b/rw76b + MO1-baxb standard conditions Protruding-mouth retinal cone cell decreased amount, abnormal
napbbrw76b/rw76b + MO1-baxb + MO2-baxa standard conditions Protruding-mouth retinal cone cell decreased amount, abnormal
napbbrw76b/rw76b + MO1-bnip1a + MO1-bnip1b standard conditions Protruding-mouth retinal cone cell decreased amount, abnormal
napbbrw76b/rw76b + MO2-baxa standard conditions Protruding-mouth retinal cone cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 25(4), Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I., The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects, 374-387, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell