FIGURE

Fig. 2

ID

ZDB-FIG-130815-31

Publication

Nishiwaki et al., 2013 - The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects

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Fig. 2

The coa Mutant Gene Encodes β-SNAP1(A) A non-sense mutation occurs at 152Y in β-snap1 gene in the coa mutant.(B) There are four zebrafish snap genes: two α-snap and two β-snap genes. β-SNAP1 has 90.8% amino acid identity to β-SNAP2, whereas β-SNAP1 has 78% and 73% amino acid identity to α-SNAP1 and α-SNAP2, respectively. In accordance with the evolution tree, zebrafish α-snap2 gene is positioned outside the human α-snap gene. Zebrafish β-snap genes seem to be duplicated after separation from human β-snap gene. sec17p is a yeast α-SNAP homologous gene. (C) Labeling of 84 hpf WT, coa mutant retinas, and coa mutant retinas expressing β-SNAP1 with zpr1 antibody (green) and GFP-tagged peripherin (magenta). Middle panels show higher magnification of the outer photoreceptor layer. Lower panels show the magenta channel. Scale bars, 50 μm (upper panels) and 10 μm (middle panels). See also Figure S2.

Expression Data

Antibody:	zpr-1
Fish:	WT napbb^rw76b/rw76b
Anatomical Term:	retinal cone cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 25(4), Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I., The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects, 374-387, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell