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ZFIN ID: ZDB-FIG-130815-30
Nishiwaki et al., 2013 - The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects. Developmental Cell   25(4):374-387 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 6
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Days 7-13

Fig. 1 Photoreceptors Undergo Apoptosis in the Zebrafish coa Mutant (A) Plastic sections of WT and coa mutant retinas at 6 dpf. ONL is absent in the coa mutant. (B) Zpr1 antibody labeling of 6 dpf WT and coa mutant retinas. All nuclei are counterstained with SYTOX Green (green). Arrowheads indicate zpr1 expression (magenta) in the CMZ of the coa mutant. (C) Plastic sections of CMZ of 6 dpf WT and coa mutant retinas. White dots indicate morphologically distinct ONL in the coa mutant. (D) Labeling of 6 dpf WT and coa mutant retinas with anti-red, blue, UV opsins, or rhodopsin antibodies (magenta); anti-green opsin antibody (green); and zpr1 (green) antibody. (E) Semithin sections of WT and coa mutant retinas at 72 hpf and 7 dpf. At 72 hpf, pyknotic nuclei were observed in the ONL in the coa mutant (arrowheads). At 7 dpf, ONL was absent in the coa mutant. INL, inner nuclear layer; IPL, inner plexiform layer; OPL, outer plexiform layer; RGL, retinal ganglion cell layer. Scale bars, 50 μm (A and B) and 20 μm (C–E). See also Figure S1.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-opn1lw1 IHC WT standard conditions Day 6 red sensitive photoreceptor cell
IHC napbbrw76b/rw76b standard conditions Day 6 Not Detected red sensitive photoreceptor cell
Ab1-opn1mw1 IHC WT standard conditions Day 6 green sensitive photoreceptor cell
IHC napbbrw76b/rw76b standard conditions Day 6 Not Detected green sensitive photoreceptor cell
Ab1-opn1sw1 IHC WT standard conditions Day 6 UV sensitive photoreceptor cell
IHC napbbrw76b/rw76b standard conditions Day 6 Not Detected UV sensitive photoreceptor cell
Ab1-opn1sw2 IHC WT standard conditions Day 6 blue sensitive photoreceptor cell
IHC napbbrw76b/rw76b standard conditions Day 6 Not Detected blue sensitive photoreceptor cell
Ab2-rho IHC WT standard conditions Day 6 retinal rod cell
IHC napbbrw76b/rw76b standard conditions Day 6 Not Detected retinal rod cell
zpr-1 IHC WT standard conditions Day 6 retinal cone cell
IHC napbbrw76b/rw76b standard conditions Day 6 ciliary marginal zone
Phenotype Details
Fish Conditions Stage Phenotype
napbbrw76b/rw76b standard conditions Protruding-mouth photoreceptor cell apoptotic, abnormal
Day 6 blue sensitive photoreceptor cell decreased amount, abnormal
Day 6 green sensitive photoreceptor cell decreased amount, abnormal
Day 6 photoreceptor cell decreased amount, abnormal
Day 6 red sensitive photoreceptor cell decreased amount, abnormal
Day 6 retinal outer nuclear layer absent, abnormal
Day 6 retinal rod cell decreased amount, abnormal
Day 6 UV sensitive photoreceptor cell decreased amount, abnormal
Days 7-13 retinal outer nuclear layer absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 25(4), Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I., The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects, 374-387, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell