|ZFIN ID: ZDB-FIG-130814-23|
Fig. 2 phox2b-deficient embryos show impaired differentiation of sympathetic neurons in the SCG.
(A–F) Lateral/oblique views of 4-dpf embryos after whole-mount ISH for th (A–C) and dbh (D–F) in control and phox2b–deficient embryos. Arrows indicate the SCG. Knockdown of phox2b by injection of a splice MO (4 ng) (MOsplice) inhibits the expression of th and dbh (B, E) which is rescued by coexpression of human PHOX2B mRNA (10 ng/μl) (C, F). Relative intensity levels of th (G) and dbh (H) expression in embryos injected with phox2b MOs that inhibit translation (MOATG) or splicing (MOsplice). Mismatched control MO (MOmm) and PHOX2B mRNA-rescue (MOsplice/PHOX2B) are also shown. Data are presented as means ± SD (***P<0.001; **P<0.01; n = 15 for each group).
|dbh||WT||control||Day 4||superior cervical ganglion||ISH|
|WT + MO3-phox2bb||standard conditions||Day 4||superior cervical ganglion||ISH|
|th||WT||control||Day 4||superior cervical ganglion||ISH|
|WT + MO3-phox2bb||standard conditions||Day 4||Not Detected||superior cervical ganglion||ISH|
|WT + MO3-phox2bb||standard conditions||Day 4||sympathetic ganglion development disrupted, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.|