ZFIN ID: ZDB-FIG-130719-15
Neal et al., 2013 - H. pylori Virulence Factor CagA Increases Intestinal Cell Proliferation by Wnt Pathway Activation in a Transgenic Zebrafish Model. Disease models & mechanisms   6(3):802-10 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
PHENOTYPE:
Fish: b1231Tg, b1232Tg, b1233Tg, tp53zdf1/zdf1; b1232Tg
Observed In: intestinal lamina propria mucosa adenocarcinoma, intestinal lamina propria mucosa small cell carcinoma, intestinal mucosa, intestine
Stage: Adult

Fig. 5 CagA expression causes phosphorylation-dependent intestinal epithelial hyperplasia and induces adenocarcinoma formation in combination with p53 loss. (A–F) H&E stained sagittal sections of adult zebrafish intestine (A) Wild-type intestine at 18 months post-fertilization (mpf) showing normal intestinal architecture, with a single layer of epithelial cells lining the mucosal folds. (B,D) b-cagA (B) and i-cagA (D) intestines at 12 mpf, displaying mucosal fold epithelial hyperplasia, dysplasia within mucosal sulci and mucosal fold fusion. (C) b-cagAEPISA intestine at 12 mpf showing normal intestinal architecture, identical to wild-type. (E) i-cagA; tp53M214K/M214K small cell carcinoma with small nests of neoplastic cells in the lamina propria (arrowheads). Inset depicts higher magnification of tumor cells; the cross marks the epithelium. (F) i-cagA; tp53M214K/M214K adenocarcinoma, poorly differentiated, invade into the lamina propria with complete disorganization of the epithelium, as shown by goblet cells randomly scattered throughout (arrowheads). (G) Summary of intestinal histological abnormalities observed in adult CagA-expressing animals as a result of a blinded histological analysis of H&E stained sections (wild-type, n=22; b-cagA, n=24; b-cagAEPISA, n=18; i-cagA, n=19; tp53M214K/M214K, n=5; i-cagA/tp53M214K/M214K, n=7). Scale bars: 25 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
b1231Tg standard conditions Adult intestinal mucosa hyperplastic, abnormal
b1232Tg standard conditions Adult intestinal mucosa hyperplastic, abnormal
b1233Tg standard conditions Adult intestine morphology, normal
tp53zdf1/zdf1; b1232Tg standard conditions Adult intestinal lamina propria mucosa adenocarcinoma poorly differentiated, abnormal
Adult intestinal lamina propria mucosa adenocarcinoma well differentiated, abnormal
Adult intestinal lamina propria mucosa small cell carcinoma neoplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.